Bacterial Load Reduction in Guheswori Sewage Treatment Plant, Kathmandu, Nepal

  • Bikram Gautam Department of Microbiology, St. Xavier's College, Maitighar, Kathmandu, Nepal
  • Anjita Rajbhanshi High Powered Committee for Integrated Development of the Bagmati Civilization (HPCIDBC), Ministry of Urban Development, Ghusewori, Kathmandu, Nepal
  • Rameshwar Adhikari Research Center for Applied Science and Technology (RECAST), Tribhuvan University, Kathmandu, Nepal



Background: Water sources such as lakes, ponds, river etc. have been continuously contaminated by the   micro organisms and chemicals. The former can pose a significant threat to human health. This work aims at detecting the bacterial load before and after the sewage treatment and hence isolating pathogens from the sewage before primary treatment and secondary treated effluent. Methods: Grab sampling (50mL sewage before primary treatment and secondary treated effluent) was performed for 20 days in the Guheswori       sewage treatment plant. The reduction in microbial load was determined through heterotrophic plate count. Pathogens were screened from the effluent obtained from the secondary treatment plant. Results: Bacterial load reduction was found to be about 48.02% on average. The observed bacterial load reduction might have been caused by bacteriophage flocculation and sedimentation. Pathogens isolated from the treated effluent were Escherichia coli, Salmonella Typhi, Enterococcus faecalis, Staphylococcus aureus, Coagulase negative Staphylococcus (CONS), Citrobacter fruendii, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris,       Pseudomonas   aeruginosa. Conclusions: It has been found that the sewage treatment plant helps to reduce the bacterial load which is, however, not capable of effluent polishing where all pathogens are killed.

 Keywords: bacterial load; pathogens; sewage treatment.

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