U, SuchitraM, KundabalaMM, Shenoy2025-07-242025-07-242006https://hdl.handle.net/20.500.14572/592Suchitra U1 , Kundabala M2, Shenoy MM3 1 Assistant Professor, Department of Microbiology, Kasturba Medical College, 2 Department of Conservative Dentistry, Manipal College of Dental Sciences, Mangalore, 3 Department of Skin and VD, K S Hegde Medical Academy, Deralakatte, MangaloreSuccess of root canal therapy depends on the complete eradication of microflora from the root canal system. A great deal of research is needed to identify and define the role of the pathogens which are involved in the pathogenesis of the periradicular diseases. This will help the endodontist to plan the best treatment by irradiation of pathogens which, in turn predict the outcome of the treatment. This article reviews the endodontic microflora, routes of microbial entry, methods to identify endodontic microbes and markers that permit the clinician to know when to conclude the treatment. Key words: Endodontic flora, Anachoresis, Culture media, Polymerase Chain Reaction, Antibiotic sensitivity testing.en-USArticle