Adhikari, RKSherchand, JBHoti, SLVishal, LAMishra, SRWeber, EJWKhanal, PRJoshi, GDumre, SPAdhikari, RNazeer, SRanabhat, KWagle, RR2026-04-292026-04-292015https://hdl.handle.net/20.500.14572/5986RK Adhikari Institute of Medicine, Maharajgunj Medical Campus, Tribhuvan University, Kathmandu, Nepal JB Sherchand Institute of Medicine, Maharajgunj Medical Campus, Tribhuvan University, Kathmandu, Nepal SL Hoti Regional Medical Research Centre, Indian Council for Medical Research, Department of Health research, Government of India, Nehru Nagar, Belgaum, Karnataka LA Vishal Regional Medical Research Centre, Indian Council for Medical Research, Department of Health research, Government of India, Nehru Nagar, Belgaum, Karnataka SR Mishra Manmohan Memorial Institute of Health Sciences, Nepal EJW Weber Fordham University, Bronx, New York, USA PR Khanal Manmohan Memorial Institute of Health Sciences, Nepal G Joshi Manmohan Memorial Institute of Health Sciences, Nepal SP Dumre World Health Organization, Country office, Nepa R Adhikari Manmohan Memorial Institute of Health Sciences, Nepal S Nazeer Regional Medical Research Centre, Indian Council for Medical Research, Department of Health research, Government of India, Nehru Nagar, Belgaum, Karnataka K Ranabhat National Public Health Laboratory, Kathmandu, Nepal RR Wagle Institute of Medicine, Maharajgunj Medical Campus, Kathmandu, NepalAbstract Introduction: Lymphatic filariasis (LF) is a neglected tropical disease, caused by Wuchereria bancrofti parasite and pose potential risk to about 1.4 billion people in 73 countries. In Nepal, 60 out of 75 districts are endemic and nearly 90% of the population is at risk and mass drug administration (MDA) has beenin place since 2003. Yet, information about the genetic diversity of W. bancrofti is largely lacking. Methods: The genetic variability of the parasites in two localities Kailali and Kapilbastu from south- western parts of Nepal were studied. Blood samples were collected at night and stained with Giemsa stain and positive archived slides were taken for the study. W. Bancrofti was individually picked under microscope and gDNA was isolated from the pooled (100 mf) samples. Short Tandem 29bp Repeats (STR) from the intronic region of Abundant Larval Transcript-2 (ALT-2) gene and haplotype mapping of the Internal Transcribed Spacer (ITS-1) region were studied. The phylogenetic trees were constructed and analyzed. Results: The analyses of STR, haplotypes and the phylogenetic trees indicated the presence of at least two genetically distinct clusters among the W. Bancrofti parasite populations in two areas. Conclusion: The study identified two genetically distinct clusters of mf in the populations. The finding of two genetic “variants” of W. bancrofti in the present study has important implications for filariasis epidemiology and control/elimination program. Keywords: Genetic variability, Lymphatic filariasis, Nepal, Wuchereria bancroftien-USGenetic variabilityLymphatic filariasisNepalWuchereria bancroftiArticle