Publication: Pseudomonas aeruginosa Isolates among Clinical Samples showing Growth in a Tertiary Care Centre: A Descriptive Cross-sectional Study
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Nepal Medical Association
Abstract
Abstract
Introduction: Carbapenems resistance due to metallo-beta-lactamase production in Pseudomonas aeruginosa is a major concern which is increasing globally resulting in limited therapeutic choices. This study aimed to find out the prevalence of Pseudomonas aeruginosa isolates among clinical samples showing growth of a tertiary care centre.
Methods: A descriptive cross-sectional study was conducted on various clinical samples which showed growth in the Department of Microbiology of a tertiary care centre between 1 September 2020 to 28 February 2021 after receiving ethical approval from the Institutional Review Committee (Reference number: 03-G/020). Convenience sampling was done. All timely received clinical specimens were inoculated and incubated at 37°C for 48 hours and identified by standard microbiological techniques. Point estimate and 95% Confidence Interval were calculated.
Results: Among 1049 clinical samples showing growth, 68 (6.48%) (4.99-7.97, 95% Confidence Interval) Pseudomonas aeruginosa were isolated. Among them, 6 (8.82%) were found to be metallo-beta-lactamase positive.
Conclusions: The prevalence of Pseudomonas aeruginosa was similar to the studies done in similar settings. As metallo-beta-lactamase production was detected among isolated species which can be spread very rapidly and may develop a problematic scenario in treatment procedures, regular surveillance along with judicious use of carbapenems should precede.
Description
Nabina Maharjan
Department of Microbiology, Lumbini Medical College and Teaching Hospital, Tansen, Palpa, Nepal
Keywords
Beta-lactamase, Multidrug resistant, Pseudomonas aeruginosa