Browsing by Author "Karkee, P"
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Publication Correlation of Bacteriuria and Pyuria among the suspected cases of Urinary Tract Infection in Kathmandu University Hospital(Institute of Medicine, 2016) Sherchan, JB; Karkee, P; Wenju, SAbstract Infection of urinary tract is one of the most important infectious diseases in the world affecting all age groups across the life span. Bacteriuria and pyuria being some of the features of urinary tract infection need to be correlated. The objective of the study is to determine correlation of bacteruria with pyuria in suspected urinary tract infection. Methods: This study was conducted at Kathmandu University Hospital, Dhulikhel between September 2014 to March 2015. During this period, 272 urine samples were collected and analyzed by microscopic examination and semi-quantitative culture technique with standard microbiological methods. Results: A total of 272 urine samples were processed in which 78(28.67%) were culture positive. Among culture positive samples significant bacteriuria (i.e. ≥10 CFU/ml) was detected in 65(83.3%) of the cases whereas 13(16.7%) of the culture positive samples showed low count significant bacteriuria (i.e. 10-10 CFU/ml). Out of 58 urine samples with significant pyuria, 48(82.75%) were culture positive. Among 214 urine samples without significant pyuria, 30(14.01%) urine samples were culture positive which was significant (P value<0.05). Conclusion: The study showed that pyuria and bacteriuria had no correlation in the suspected cases of urinary tract infection. According to our findings, it is necessary to culture urine and also perform microscopic examination for the correct laboratory diagnosis of urinary tract infection Keywords: urinary tract infection, culture, bacteriuria, pyuria, microscopyPublication Mobile Phone: A Possible Vector of Bacterial Transmission in Hospital Setting(Kathmandu University, 2017) Karkee, P; Madhup, SK; Humagain, P; Thaku, N; Timilsina, BABSTRACT Background Mobile phones of Health Care Workers (HCWs) are capable of harbouring microorganisms that can potentially cause nosocomial infections. Frequent movement of hospital staff inside and outside the hospital can augment bacterial transmission inside hospital and even to the community. Objective To screen the mobile phones of Health Care Workers for potential pathogens and perform microbiological study of the isolates. Methods A cross sectional study was conducted in 124 Health Care Workers of different departments of tertiary care hospital. Swabs were taken from their mobile phones, processed immediately and identified and their antibiotic suceptibility pattern was studied. Results This study revealed that bacterial growth was positive for pathogenic organisms in 89 out of 124 (71.8%) mobile phones out of which 33 (82.5%) were of male and 56 (66.7%) of female. However, there was no significant association between gender and isolation of pathogens. Single pathogen was isolated in 74 (59.7%) of mobile phones and multiple pathogens were isolated in 15(12.1%). Amongst them, most common pathogen isolated was Coagulase Negative Staphylococcus (CoNS) (56.7%) followed by Micrococcus spp., Escherichia. coli, Enterobacter spp., Acinetobacter spp., Staphylococcus. aureus, Klebsiella spp. and Enterococcus spp. Most of the Gram Positive Cocci (GPC) were sensitive to Vancomycin (81.9%) and Ciprofloxacin (88%) while were resistant to Penicillin(83.1%). For Gram negative bacteria sensitivity to ciprofloxacin ranged from 83.33% to 100% . Conclusion Mobile phones are possible vectors of bacterial transmission and therefore are constant threat to the lives of already seriously ill patients as well as healthy individuals visiting the hospital. So it is recommended to make infection control guidelines which target the use of suitable disinfectants to avoid mobile phone contamination. KEY WORDS Antibiotics, Health care workers, Mobile phones, Nosocomial infection