Browsing by Author "Lewis, Leslie Edward"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Publication Neutrophil Counts in Hospitalised VLBW and ELBW Neonates – A Prospective Observational Study(Perinatal Society of Nepal (PESON), 2024) Raja, Adusumilli Venkata Naga; Purkaystha, Jayashree; B, Sandesh Kini; Lewis, Leslie Edward; Bhat Y, RameshAbstract: Introduction: Early onset sepsis (EOS) is an important cause of morbidity and mortality in new born babies with diagnostic challenges. The role of neutrophil count in EOS in neonates has been studied extensively but with varying results. Methods: This was a prospective observational study conducted among VLBW neonates admitted in our neonatal intensive care unit (NICU) between October 2014 to June 2016. Complete blood count (CBC) was carried out for all neonates enrolled in the study. Our primary objective was to study the neutrophil count in very low birth weight (VLBW) neonates. Our secondary objectives were to study the total leucocyte count (TLC) along with neutrophil count on day one in neonates with EOS and to study neutrophil count in VLBW neonates born to mothers with pregnancy induced hypertension (PIH). Results: Among 175 neonates, 92(52.6%) were males and 83(47.4%) were females. The mean birth weight was 1105 ± 241 gm. Neutropenia was observed among 10 neonates on day 1 with a mean neutrophil count of 791.2 ± 315.8/µL. Neutrophilia was observed among 27 neonates on day 1 with a mean neutrophil count of 9054.2±3361.4/ µL. Four out of 10 neonates with neutropenia on day 1 had EOS in comparison with 18 out of 165 neonates without neutropenia and the results were statistically significant (p=0.024). However, there was no significant association between PIH and neutrophil count. Conclusions: Neutrophil count is a valuable indicator of sepsis among VLBW neonates. Low neutrophil count has significant association with EOS among VLBW neonates.Publication Role of Neutrophil CD64 in the Diagnosis of Neonatal Sepsis(Nepal Paediatric Society (JNPS), 2022) Moideen, Adeel; Barche, Apurv; Andrade, Sneha Jaganathan; Verma, Aditya; Lewis, Leslie Edward; Purkayastha, Jayashree; Hadi, Ayesha ErumAbstract: Introduction: Neutrophil surface CD64 (Cluster of differentiation 64), the highaffinity Fc receptor, is quantitatively up-regulated during infection and sepsis. The diagnostic utility of NCD64 as a reliable marker of neonatal sepsis has not been explored so far. Hence this study has been conducted to compare NCD64 with other currently used infection markers including total leucocyte count, platelet count, absolute neutrophil count (ANC), band:neutrophil ratio and highly sensitive C reactive protein (hs-CRP). Methods: Consecutively born neonates between March 2014 to November 2014 were enrolled with documented sepsis (n = 81), clinical sepsis (n = 35), and no sepsis (n = 87). NCD64 was analyzed by flow cytometry. Results: Sepsis episodes had a higher median CD64 index of 10.35 (Range: 15.88, 6.87) as against 2.97 (Range: 5.53, 1.64) in the control group (p < 0.001). The percentage of NCD64 positive cells was also significantly higher in the sepsis group compared to the control group (63.90 ± 2.67 vs 15.07 ± 1.95; p = 0.001). In the ROC curve analysis NCD64, percentage of NCD64 positive cells had the highest AUC (AUC-0.914) using a cutoff of 28.01%, followed by CD64 mean fluorescence intensity (MFI) with an AUC of 0.850 using a cutoff of 5.54. NCD64 was significantly elevated in the groups with documented and clinical sepsis (p < 0.001). Conclusions: NCD64 is a highly sensitive marker for neonatal sepsis. Prospective studies incorporating NCD64 into a sepsis scoring system are warranted.