Browsing by Author "Sherchand, JB"
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Publication Antimicrobial susceptibility pattern and serotyping of Streptococcus pneumoniae isolated from Kanti Children Hospital in Nepal(Kathmandu University, 2010) Rijal, B; Tandukar, S; Adhikari, R; Tuladhar, NR; Sharma, PR; Pokharel, BM; Gami, FC; Shah, A; Sharma, A; Gauchan, P; Sherchand, JB; Burlakoti, T; Upreti, HC; Lalitha, MK; Thomas, K; Steinhoff, MAbstract Background: Invasive pneumococcal disease is a significant cause of morbidity and mortality worldwide and it is a major cause for childhood deaths in Nepal. Objectives: The aim of this study was to establish the antimicrobial susceptibility pattern of Streptococcus pneumoniae and perform serotype responsible for pneumococcal disease in Nepal. Materials and methods: All together 3774 children from 2 to 60 months who fulfilled the enrollment criteria for suspect of bacterial pneumonia, sepsis or meningitis were enrolled for etiologic studies of severe illness. During the study period 60 isolates of Streptococcus pneumoniae were isolated and the antimicrobial susceptibility testing and serotyping were performed. Results: The study showed that 24 (52.17%) isolates were resistant to Cotrimoxazole, 3 (6.5%) isolates were intermediately resistant to Penicillin but no Penicillin resistant strains were isolated. The 1 (2.17%) isolate was recorded as Erythromycin and Chloramphenicol resistant and only 1 (2.17%) isolate was found intermediately resistant to Cefotaxime. Of the 60 isolates, serotyping result was available only for 46 isolates. The most common serotypes were serotype 1 (27. 65%) followed by serotype 5 (19.14%) and serotype 4 (8. 5%) respectively followed by serotype 39, 23F, 7F, 19B, 12A, 14, 18F, 6B, 32, 16, 19F and 25F. Conclusions: Alarming level of Cotrimoxazole resistance demands revision of pneumonia treatment policy in Nepal and rising tendency of other drug resistance against Streptococcus pneumoniae showed use of these drugs for the treatment of meningitis, pneumonia and other serious infections needs extended research. The common serotype 1, 5 and 4 need to be incorporated in pneumococcal vaccine to immunise children in Nepal. Key words: Antimicrobial susceptibility, Streptococcus pneumoniae, Serotyping, NepalPublication Assessment of Health Systems in Relation to Interface Between Malaria Control Programs and Health System Strengthening: Comparative Study Between Nepal and Viet Nam(Institute of Medicine, 2015) Oraha, H; Sherchan, JB; Pokhrel, BM; Hirayama, T; Huy Nam, Vu; Sherchand, JBAbstract Introduction: Malaria control has been a major health issue with high priority in endemic countries and various efforts have been made with the support of foreign assistant partners. In order to implement efficient and sustainable control, integration of the control program into general health system or effective interactions between them is one of the important strategies. Methods: Studies were conducted in Nepal and Viet Nam. Information obtained from document reviews, interviews, and field surveys were analyzed from the viewpoint of interface between malaria control program and the health system in accordance with six building blocks of a health system, with special emphasis on good practices and challenges in the implementation of the malaria control program. Results: Among good practices, strong government commitment towards the control programs to strengthen facilities and capacity of health workers at the primary level, utilization of health volunteers, setting up mobile team and intensified education for residents were noteworthy. Key challenges mainly involved remote areas. Introduction of malaria due to population movement and the emergence of new endemic areas have become growing issues. While strengthening of the vertical health program appeared to have some impact on the general health system, particularly at the primary level, dissociation between the vertical control program and horizontal general health system still remains. Conclusion: It is crucial to implement an effective and equitable malaria control program that responds to these existing challenges and can create a sustainable health system. Addressing these issues will lead to further strengthening of the health system there and eventually lead to the effective implementation of various health programs. Keywords: malaria control, health system, Nepal, Veit NamPublication Clinical observations among patients with Dengue Fever in Nepal(Institute of Medicine, 2014) Neupane, B; Sherchand, JB; Pandey, BDAbstract Introduction: Dengue is an arthropod-borne viral illness that has become a serious public health threat in Nepal. The disease has been reported frequently during the post monsoon period in the southem lowlands of the country. Studies relating to sero-prevalence of dengue have been performed regularly in Nepal but the most frequent clinical observations among the patients with dengue fever have not been well documented. We report thrombocytopenia (74%), headache (44%) and myalgia (44%) among the febrile cases as the most frequent features of the 287 dengue cases identified by rapid immunochromatographic tests and ELISA during 2010 and 2011. These findings are potentially helpful for the clinicians to predict dengue in the early stage in those areas where dengue has been endemic, thus minimizing the significant morbidity and mortality of the disease. Keywords: Dengue, disease, clinical features, NepalPublication Co-existence of aminoglycosides and β-lactam-resistant Escherichia coli phenotypes in a Tertiary care center of Nepal(Institute of Medicine, 2015) Shrestha, B; Tada, T; Shrestha, S; Katte, HPl; Ohara, H; Kirikae, T; Rijal, BP; Sherchand, JB; Pokhrel, BMAbstract Introduction: Multidrug-resistant Escherichia coli isolates conferring simultaneous resistance to both aminoglycosides and β-lactam drugs have serious implications for clinicians worldwide. This study was designed to evaluate the co-existence of various β-lactamases in aminoglycoside- resistant Escherichia coli amongst hospitalized subjects in a tertiary care center of Kathmandu, Nepal, between December 2013 and December 2014. Methods: Standard microbiological techniques were used for isolation and identification of the isolates. The antimicrobial susceptibility of bacterial isolates was determined following Clinical and Laboratory Standard Institute recommended Kirby-Bauer Disc Diffusion method. The defining criterion in this study for an isolate to be MDR, resistance to at least one agent in three or more than three different structural classes was taken. Results: Among 302 MDR E. coli isolates, 174 (58.0 %) were resistance to gentamicin and 138 (46.0 %) were resistance to amikacin. Maximum aminoglycoside-resistant 9/11(82.0%) strains were isolated from body fluids followed by 7/10 (70.0%) from bile, 6/9 (67.0%) from blood and 2/3 (67.0%) from tissue. Out of 174 aminoglycosides-resistant E. coli isolates, the simultaneous occurrence of Extended-spectrum- b- lactamase (ESBL) and AmpC β–lactamaseswas noted in 13.0 % isolates and Metallo-β-lactamase (MBL) and AmpC β–lactamasesin 8.0 % isolates. None E. coli isolates were positive for all 3 types of β–lactamases in combinations. In amikacin- resistant isolates, ESBL+ AmpC observed in 12% and MBL+AmpC seen in 10% isolates. Conclusion: Our results show a high frequency of aminoglycoside- resistance phenotypes. Strict application for appropriate use of antimicrobials in medical settings should be essential to minimize the emergence of multidrug-resistance among E. coli in hospitalized patients. Keywords: Aminoglycoside-resistant E. coli, Amp C β–lactamases, Metallo-β-lactamase, MDR E.coliPublication Detection of Cryptosporidium, Giardia, fecal Indicator bacteria, and total bacteria in commercial jar water in the Kathmandu Valley, Nepal(Institute of Medicine, 2015) Malla, B; Ghaju, Shrestha R; Bhandari, D; Tandukar, S; Shrestha, S; Yoshinaga, H; Inoue, D; Sei, K; Nishida, K; Tanaka, Y; Sherchand, JB; Haramoto, EAbstract Introduction: Jar water is a convenient and common source of drinking water in the Kathmandu Valley. However, studies including detailed microbial analyses of this source of potable water are lacking. In this study, jar water samples were examined for the occurrence of Cryptosporidium, Giardia, fecal indicator bacteria, and total bacteria. Methods: Thirty different brands of jars were collected in September 2014. Escherichia coli and total coliforms were determined using a Colilert reagent. Ten of the 30 brands were selected to test for Cryptosporidium, Giardia, and total bacteria. Bacterial DNA extraction from water samples was performed using the Cica Geneus DNA Extraction Kit, followed by quantitative polymerase chain reaction (qPCR) targeting the 16S rRNA gene of bacterial DNA. Protozoan detection was accomplished by concentrating the samples using the electronegative membrane vortex method. followed by immunomagnetic separation and fluorescent staining. Results: E. coli was detected in 10% of the samples, with a maximum concentration of 2 most probable number (MPN)/100 ml., whereas total coliforms were detected in 97% of the samples, with a maximum and mean concentration of 7.3 x 102 and 3.8 x 10 MPN/100 ml., respectively. Total coliforms concentrations in 40% of the samples ranged from 10 to 10 MPN/100 ml. Cryptosporidium and Giardia were not detected in any of the tested samples. Concentrations of total bacteria in the samples ranged from 10 to 10 cells/100 ml. Conclusions: Ninety-seven percent of the jar water brands were unsuitable for drinking without proper treatment based on the guideline values of the National Drinking Water Quality Standards (NDWQS) of Nepal. There is no guideline value for total bacteria in NDWQS however, high concentrations can be indicative of poor control on regrowth of bacteria and recontamination or inefficient water treatment methods. Keywords: Cryptosporidium, Giardia, Jar water, qPCR, Total bacteriaPublication Earthquake disaster-associated health effects and the need for improved preventive measures(Institute of Medicine, 2015) Sherchand, JBNA.Publication Enteric Opportunistic Parasitic Infections Among HIV- Seropositive Patients in Kathmandu, Nepal(Kathmandu University, 2012) Sherchan, JB; Ohara, H; Sakurada, S; Basnet, A; Tandukar, S; Sherchand, JB; Bam, D SABSTRACT Background Enteric opportunistic parasitic infections are the major source of diarrheal disease in developing countries mainly in Human Immunodeficiency virus (HIV) infected patients. Objective The study was to detect enteric parasites causing diarrhea and their association with immune status in HIV-seropositive patients. Methods The present study was conducted in Dirgh-Jeevan Health Care Research Center and Tribhuvan University Teaching Hospital, Public Health Research Laboratory, Kathmandu, Nepal between June 2010 and May 2011 involving 146 Human Immunodeficiency virus (HIV) positive patients. Serostatus from these patients were detected by Enzyme Linked Immunosorbent assay. CD4+ T cell counts were done by flow cytometry. Stool was examined for enteric parasites by microscopy with special staining methods. Results A total of 146 HIV sero-positive patients with and without diarrhea age between 20 to 45 years were included in the study. Of the 146 patients, the protozoan parasitic infection was found in 30.13% (44/146). Out of 146 patients, 78 had diarrhea in which parasitic infection was 39 (50%) and 7.35% (5/68) protozoal parasites positive cases did not have diarrhea. A significant difference (p<0.05) was observed in the level of infection of intestinal protozoan between the HIV seropositive with diarrhea and HIV-seropositive without diarrhea. Out of 43 patients whose CD4+ T cells were <200/μl, 29 (67.4%) had opportunistic parasitic infection whereas out of 103 patients whose CD4+ T cells were ≥200/μl, only 15 (14.56%) had opportunistic parasitic infection (P < 0.05). Conclusion Enteric opportunistic parasitic infections were detected in 30.1% among HIV- seropositive patients and low CD4+ T count indicated high enteric opportunistic infection. Early detection of enteric parasitic infections will help in the management and to improve the quality of life for HIV-infected individuals. KEYWORDS Diarrhea, HIV, Opportunistic parasitesPublication Governing the medical research by ethical norms, a challenge !(Institute of Medicine, 2015) Sherchand, JBNA.Publication Human Resources for Health (HRH) and challenges in Nepal(Institute of Medicine, 2013) Sherchand, JBNA.Publication Identification of Taenia from child of 6 year old(Institute of Medicine, 2015) Sah, R; Khadka, S; Sherchand, JB; Tandukar, S; Bhandari, D; Shrestha, L; Rijal, B; Pokharel, BMNA.Publication Intestinal parasitic infection among children less than five year of age visiting Children’s Hospital of Kathmandu(Institute of Medicine, 2018) Shrestha, S; Raya, S; Shrestha, L; Parajuli, K; Sherchand, JBAbstract Introduction: Intestinal parasitosis is a worldwide concern and cause significant morbidity and mortality, particularly in developing countries like Nepal. The current study aims to identify the status of intestinal parasitosis among children less than five years of age visiting Kanti Children Hospital. Methods: Hospital based cross sectional study was conducted from January 2017 to December 2017 among children less than 5 years of age visiting Kanti Children’s Hospital. Parasites were detected by using standard microbiological procedures. Results:Among a total of 770 stool specimens collected, intestinal parasites were detected in 7.4% cases. Children of age group 49-60 months were found to be infected more often followed by 37-48months with higher frequency seen in summer season in the month of May and June. Altogether five species of parasites were detected, among which three were protozoan and two were helminths. The most common parasite identified wasGiardia lamblia (4.3%) followed by Entamoebahistolytica(2.2%) andHymenolepis nana(0.5%). Conclusion:The study indicated that there is still burden of parasitic infection in Nepal among children. Hence, there is a need to incorporate effective prevention and control strategies including healtheducation and environmental hygiene. Keywords: Intestinal parasitosis, parasites, Entamoeba histolytica, Giardia lamblia, children NepalPublication Intestinal Parasitic Infection among School children in Chitwan district of Nepal(Institute of Medicine, 2015) Bhattachan, B; Panta, YB; Tiwari, S; Thapa Magar, D; Sherchand, JB; Rai, G; Rai, S KAbstract Introduction: Parasitic infection occur in children of all ages living under poor sanitation, eating unhealthy food and drinking water. Study was conducted from January to June, 2012 at Saktikhor in Chitwan district of Nepal. The aim for this study was to determine prevalence rate of intestinal parasites among children (< or =18 aged) group. Methods: 296 stool samples were collected in dry, clean and screw capped plastic container. Stool samples were preserved with 10% formalin, transported to Shi-Gan Health Research Laboratory then samples were examined microscopically by formal-ether sedimentation technique. Results: Overall, Positive rate was 23.3% (69/296). There was no significance difference in two genders boys 21.8% and girls 24.8%, (p=0.39). Positive rate in Tibeto-Burman was highest 23.2% followed by Indo-Aryan 22.1% and Dalit 29.6%, (p=0.80). In drinking water, parasitic infection rate in well water was found higher 29.9% than tap water 21.9%, (p=0.263). Positive rate in no drug (anti-parasitic) user was found higher 32.1% than drug user 16.0%, (p=0.002). Age groups between 0-5 years Children was 26.9% highest in positive rate followed by 6-12 years 25.15% and 13-18 years 15.2%, (p=0.35). Altogether 10 species were identified. Taenia spp was most common found 21.0% followed by Entamoeba coli (17.0%), Giardia lamblia (17.0%), Endolimax nana (13.0%), Ascaris lumbricoides (11.0%) Entamoeba histolytica/ dispar (11.0%), Trichuris trichiura (4.0%), Hymenolepsis nana (3.0%), Blastocystis hominis (3.0%), and Hookworm (1.5%). Conclusion: Children should focus on improvement of sanitation practice, periodic administration of anti-parasitic drug and safe drinking water. Keywords: Children, Chitawan, Intestinal Parasites, NepalPublication Intestinal Parasitic Infection with Nutritional Status among Orphan Children in three cities of Nepal: Prevalence and Associated risk factors(Institute of Medicine, 2018) Chaudhary, RB; Bhattachan, B; Thapa Magar, D; Dhami, RS; Karki, AB; Sherchand, JBAbstract Introduction: Intestinal parasitosis and malnutrition are common health problems among children in developing countries like Nepal. The aim is to determine the prevalence of intestinal parasites along with nutritional status in orphan children and find out its risk factors. Methods: National Institute of Tropical Medicine and Public Health Research Laboratory, Kathmandu, Nepal had approved this research. Cross sectional study was conducted from January to June, 2014. Stool samples were collected in clean wide mouthed plastic container from ≤ 15 year’s children of different orphanages in Chitwan, Kaski and Kathmandu Valley of Nepal. Socio-demographic information was collected using a structured questionnaire. Formalin-ether-sedimentation technique was done for detection of cyst and eggs of parasites. For nutritional status, anthropometric measurements were taken at the time of interview. Results: In total of 309 orphan children (male: 167, female: 142), 20.3% (63/309) had some kinds of cysts or eggs of parasites.Altogether six different parasites were detected; in which infection rate of Entamoeba coli was highest 5.2% (16/309), followed by Giardia lamblia 3.6% (11/309), Entamoebahistolytica 3.6% (11/309), Trichuris trichiura 2.9% (9/309), Hymenolepis nana 2.3% (7/309) and Ascaris lumbricoides 1.2%(4/309). 1.6% had multiparasites. The prevalence of malnutrition was found to be 16.1% (50/309). Among those malnutrited children, infection rate of parasites in obesity and thinness children were found 31.3% (5/16), and 25.8% (8/31) respectively. Conclusion: Intestinal parasites and malnutrition are associated each other in orphan children with its risk factor like age, sex and other behavioral activities. Prevalence of protozoa was found higher than helminthes. Obesity was predominance in malnutrition children. Keywords: Intestinal parasites, Nutritional status, Orphan-children, Kathmandu, Kaski, Chitwan, NepalPublication Leptospirosis: An Emerging Infectious Disease in Nepal(Institute of Medicine, 2016) Bhattachan, B; Bhattacharya, A; Sherchan, JB; Dhoubhadel, BG; Sherchand, JBAbstract Introduction: The aim of this study was to determine prevalence of Leptospira spp in Japanese Encephalitis negative cases, as well as its demographic and geographical picture in Nepal. Methods: The Nepal government along with World Health Organization (WHO) country office Nepal had approved this surveillance project, which conducted from 2007 to 2008 throughout 47 districts in Nepal. JE was confirmed from Cerebrospinal fluid (CSF) samples using ELIZA Method whereas Leptospiraspp was identified by using Latex Agglutination Test kit method in Nepal Public Health Laboratory (NPHL), Kathmandu, Nepal. Results: Among 2690 Acute Encephalitis Surveillance, the lumber puncture (LP) was done in 2145 patients. Among those tested, JE positive was confirmed in 771 patients. LP was not done in 381 cases. Among 993 JE negative cases, positivity rate of Leptospira spp was reported 41.8% (416/993) . There was more male preponderance 63.5% (264/416) However, it was not statistically significant (p = 0.713) . In terms of age distribution, those above 15 years of age were 64.5% (268/416) while those below 15 years of age were 35.5% (148/416), (p = 0) In eco- region, the rate of infection was highest in Terai region at 53.2% (222/416) followed by Mountain region at 45.2% (188/416) and Himalayan at 1.4% (6/416), (p = 0.005) In terms of seasonal distribution, the rate of infection in autumn was at 44.2% (188/416) followed by summer at 40.1% (167/416), spring at 9.6% (40/416) and winter at 6.1% (25/416) (p = 0) Conclusions: Nepal government cannot neglect the increasing possibility of outbreaks of Leptospira spp in different parts of the country, so this study recommends for surveillance of this infection to prevent future outbreaks in Nepal Keywords: Leptospira, Leptospirosis, JE, NepalPublication Mission of Institutional Review Board/ Ethical Review Committee(Institute of Medicine, 2017) Sherchand, JBNA.Publication Molecular epidemiology of Rotavirus diarrhea among children in Nepal: Emergence of G12 and G9 strains(Institute of Medicine, 2013) Sherchand, JB; Tandukar, S; Sherchan, JB; Gurung, S; Dhakwa, JR; Bichha, RP; Mahaseth, CAbstract Introduction: Rotavirus is the leading cause of diarrhea and dehydration among infants in both developed and developing countries. The primary objective of this study was to find the magnitude of rotavirus disease burden and genotypic variations of rotavirus. Methodology: Questionnaires and stool samples were collected from 1003 enrolled children under 5 years of age attending tertiary care Children’s Hospital with acute watery diarrhea during January to December 2012. Rotavirus in stool samples was detected by Enzyme Immuno Assay (EIA) and strains detected from rotavirus positive samples were genotyped by Reverse-Transcription Polymerase Chain reaction (RT-PCR). Results: Among these, 356 (35.4%) cases were positive for rotavirus by EIA, among the positive cases, 344 samples underwent genotyping by RT-PCR. Rotavirus positive cases were predominant in children who were admitted to the hospital which was 37.8% (115 out of 336). Overall G12 was the most prevalent genotype (52.3%), followed by G1 (17.7%), G2 (10.17%) and G9 (8.1%). The P types identified were P[6] (55.23%), P[8] (20%), and P[4] (12.5%). Conclusion: The study reveals that rotavirus gastroenteritis accounted for more than one-third of all cases of acute diarrhea. Use of rotavirus vaccines may reduce of high burden of rotavirus diarrhea in children. Emergence of G12 and G9 strains proves the immediate need of vaccine in Nepal. Keywords: Molecular epidemiology, rotavirus, NepalPublication Nobel discoveries from isolates of multidrug resistant Acinobacter baumannii and multidrug resistant Escherichia coli in Nepal(Institute of Medicine, 2017) Sherchand, JBNAPublication Paragonimiasis: First autochthonous case report from Nepal(Institute of Medicine, 2016) Sah, R; Khadka, S; Sherchand, JB; Parajuli, K; Shah, NP; Mishra, SK; Sharma, S; Shrestha, L; Basnet, S; Tandukar, S; Bhandari, D; Yadav, SR; Kattel, HP; Pokhrel, BM; Rijal, BNA.Publication Phenotypic characterization of multidrug-resistant Acinetobacter baumannii with special reference to metallo-β-lactamase production from the hospitalized patients in a tertiary care hospital in Nepal(Institute of Medicine, 2015) Shrestha, S; Tada, T; Shrestha, B; Ohara, H; Kirikae, T; Rijal, BP; Pokhrel, BM; Sherchand, JBAbstract Introduction: Acinetobacter baumanniiis an important cause of nosocomial infection and has been associated with a wide variety of illnesses in hospitalized patients, especially patients in the intensive care units. The emergence of carbapenem-resistant clones of A. baumannii has been the most serious problem worldwide. After the carbapenem resistant clones have emerged, leaving the hope of treatment of A. baumannii infection is by the last resort of antibiotics such as tigecycline, polymyxin-B and colistin. The purpose of this study is to determine the antibiotic resistance patterns of A. baumannil isolates, prevalence of multidrug resistance, extended spectrum beta lactamase production and metallo-beta lactamase production. Methods: This is a prospective study conducted at the department of Clinical Microbiology. Tribhuvan University Teaching Hospital, from December 2013 to September 2014. Ethical approval was taken from the Institutional Review Board of Institute of Medicine. Two hundred and forty six Acinetobacterisolates were identified by standard microbiological testing. Antimicrobial susceptibility testing was performed by Kirby Bauer method as per the CLSI guidelines. Multidrug resistance was determined. ESBL production was detected by combination disc method and confirmed by Clinical and Laboratory Standerd Institute confirmatory test. MBL production was detected by using imipenem and imipenem/EDTA disc. Result: All 122 Multidrug-resistant A. baumannii isolateswere resistant to majority of the drugs used. All the isolates were completely sensitive to polymyxin B, colistin and tigecycline only. Fifteen (12.29%) isolates of A. baumannii were extended spectrum beta-lactamase producers and 50 (40.98%) were metallo-beta-lactamase producers. Multidrug resistance was common in A. baumannii Conclusion: Multidrug resistance in A. baumannii is becoming more common ESBL and MBL production should be promptly detected and reported to control the spread of resistant phenotypes to other individuals. Keywords: Acinetobacter baumannii, ESBL, MBL, multidrug-resistancePublication Quality of life of people living with lymphoedema: A cross sectional community based study in selected districts of Nepal(Institute of Medicine, 2014) Adhikari, RK; Sherchand, JB; Mishra, SR; Ranabhat, K; Wagle, RRAbstract Introduction: Lymphatic Filariasis (LF) is the second most common cause of physical disability worldwide with 40 million people chronically disabled by the disease and about twice that number suffering from covert lymphatic changes or kidney diseases. The most common chronic manifestations of the disease are lymphoedema and hydrocele. The objective of this study is to evaluate the quality of life (QOL) of lymphoedema patients in Nepal. Methods: We sought the help of female community health volunteers, health workers working in peripheral health centers in selected sentinel surveillance sites in listing out the names of patients with lymphoedema. The researcher obtained details of 205 cases of lymphoedema (49 cases of Elephantiasis and 156 cases of hydrocele) in three districts (Dhading 74, Kapilbastu 78, Kailali 53). A total of 205 healthy individuals were selected from the same locality (Dhading 68, Kapilbastu 66, Kailali 71) for comparison. World Health Organization Quality of Life questionnaire brief version (WHO QOL-BREF) was used for data collection. Results: The mean age of the respondents was 45.95 years with a standard deviation of 17.96 years. In four domains, the physical domain scored highest (14.28±2.52) and the environmental domain scored lowest (12.19±1.69). Patients with lymphoedema had significantly low QOL scores in physical (p<0.001), psychological (p<0.001), social relationship (p<0.001), and environmental (p<0.001) compared to their healthy counterparts. Similarly, the overall QOL score was lower in patients with lymphoedema than in healthy control, and it was statistically significant (p<0.001). Conclusion: This study reports significantly low QOL scores in all four domains in patient with lymphoedema denoting low quality of life compared to their healthy counterparts. It is recommended that the current Lymphatic Filariasis elimination program of Nepal should focus to initiate morbidity management program specifically to patients with lymphoedema and hydrocele. Keywords: Lymphoedema, filariasis, quality of life, cross sectional studies, Nepal