Publication: Grafts containing human amniotic epithelial cells reducehyperactivity in open-field and digital photo-actometer afterTrimethyltin chloride lesion
Date
2010
Article Type
Original Article
Journal Title
Journal ISSN
ISSN (Print) : 1993-2979 | ISSN (Online) : 1993-2987
Volume Title
Pages
Pages: 24 - 30
Publisher
Institute of Medicine
Abstract
Abstract
Introduction: The aim of present study is to provide adequate numbers of cells to appropriate sites for useful cellular replacement to overcome the functional deficits caused by the Trimethyltin chloride lesion.
Methods: The effect of Transplanted Human Amniotic Epithelial (HAE) cells in hippocampus after Trimethyltin chloride lesion was tested on Open-field activity and Digital Photoactometer during different time duration. Hippocampal disorder was induced by the intra peritoneal administration of Trimethyltin chloride (TMT) (Sigma chemicals, U.S.A) at a single dose of 7.5 mg/kg body weight or two divided doses of 3.75 mg/kg body weight for two days. Human Amniotic Epithelial cells were isolated from placenta obtained from uncomplicated elective caesarian. Using standard co-ordinates Human Amniotic Epithelial cells were transplanted at four sites of hippocampus.
Results: In present study, we observed that the transplanted Human Amniotic Epithelial cells can aid in the partial recovery of hyperactivity induced by the Trimethyltin chloride lesion in Open-field activity and Digital Photoactometer.
Conclusions: The present study concludes that the Human Amniotic Epithelial cells may be used as a suitable donor tissue to alleviate various degenerative diseases in animal model before the clinical trial in humans, who are suffering from various degenerative diseases.
Keywords: Digital photo-actometer, human amniotic epithelial cells, open field test, transplantation, trimethyltin chloride
Description
K.V Omprakash
Department of Anatomy, Hassan Institute of Medical Sciences, Hassan, Karnataka, India
R. Muthusamy
Department of Anatomy, S.R.M. Dental College, Deemed University, Ramapuram, Chennai, India
B. Devanand
Department of Anaesthesia, Vijayanagar Institute of Medical Sciences, Bellary, Karnataka, India
Nagaraja Puranik
Department of Physiology, Karnataka Institute of Medical Sciences,Hubli, Karnataka, India
M. Mallikarjun
Department of Anatomy, Vijayanagar Institute of Medical Sciences, Bellary, Karnataka, India
Keywords
Digital photo-actometer, human amniotic epithelial cells, open field test, transplantation, trimethyltin chloride
Identifier
https://doi.org/10.59779/jiomnepal.486