Publication: Prevalence of asymptomatic bacteriuria in school going children in Pokhara valley
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Date
2007
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Publisher
Kathmandu University
Abstract
Objectives: There were mainly two objectives of the study. One was to detect bacteriuria in school going
children in Pokhara valley and the other was to identify the causative organisms in various age groups in
children.
Materials and methods: This study was carried out in the Microbiology laboratory of The School of
Pharmaceutical and Biomedical Sciences, Simalchaur, Pokhara University, Nepal. A total of 502 urine samples
of 5 to 13 years children from different schools of Pokhara valley were screened to see asymptomatic bacteriuria
during January 2005 to June 2005. A sterile wide mouth container was given to each student to collect mid-
stream urine samples. All the urine samples were transported to the Microbiology laboratory within half an hour
to one hour. The samples were processed for microscopical examination to observe for turbidity and the
presence of protein and sugar by dipstick method, microscopical examination to see pus cells, RBCs, epithelial
cells, casts and crystals, culture of urine samples on Blood agar and MacConkey agar to identify the potential
pathogens. The antibiotic sensitivity test was performed for those bacteria which were grown on culture. The
colony count was evaluated and organisms were identified by biochemical tests.
Result: Out of 502 samples, 7(1.39%) samples grew the bacterial pathogens that are responsible to cause
urinary tract infection. Among them Escherichia coli 4(57.14%) was the predominant bacterial pathogen.
Conclusion: Study of asymptomatic bacteriuria is important as found in the present study in which Escherichia
coli was the most frequently incriminated as the causative agents.
Key words: Asymptomatic bacteriuria, Escherichia coli, children
Description
Jha BK1 , Singh YI21 Lecturer, 2 Professor and Head, Department of Microbiology, Manipal College of Medical Sciences, Pokhara