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Molecular Investigation of Dengue Virus Serotypes in the Dengue Outbreak of 2022 in Nepal

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creativeworkseries.issn1812-2027
dc.contributor.authorKatuwal, N
dc.contributor.authorShrestha, A
dc.contributor.authorRanjitkar, U
dc.contributor.authorJakibanjar, S
dc.contributor.authorMadhup, SK
dc.contributor.authorTamrakar, D
dc.contributor.authorShrestha, R
dc.date.accessioned2026-01-08T07:45:08Z
dc.date.available2026-01-08T07:45:08Z
dc.date.issued2024
dc.descriptionKatuwal N,1-3 Shrestha A,1-3 Ranjitkar U,1,3 Jakibanjar S,1,3 Madhup SK,4 Tamrakar D,1,3,5 Shrestha R1-3,6 1Center for Infectious Disease Research and Surveillance 2Molecular and Genome Sequencing Research Lab 3Research and Development Division 4Department of Microbiology 5Department of Community Medicine 6Department of Pharmacology Dhulikhel Hospital, Kathmandu University Hospital Kathmandu University School of Medical Sciences Dhulikhel, Kavre, Nepal
dc.description.abstractABSTRACT Background Dengue, a viral infection highly prevalent in tropical regions, exhibits local variations in risks that are influenced by a combination of climatic, socioeconomic, and environmental factors. It is caused by four distinct, yet closely related serotypes of the dengue virus. Objective To identify the different serotypes of dengue virus responsible for the 2022 outbreak in Nepal, where dengue has been prevalent since 2006 but with limited availability of molecular information on the serotypes. Method Serum samples from suspected dengue patients visiting Dhulikhel Hospital were analyzed using Dengue Rapid Test, for the presence of IgG/IgM antibodies or NS1 Ag. The positive samples were stored at -80⁰C, and 89 samples were selected for further analysis. RNA was extracted from those positive samples and RT-PCR was performed to identify the serotypes present. Result A higher percentage of sero-positivity was observed in females(52%) compared to males. Positive cases were distributed in 14 different districts, with the highest percentage(58.4%) in Kavre. RT-PCR, of 53 out of 89 serologically positive samples, by RT-PCR revealed that DENV1 was the predominant, followed by DEN3(24.5%) and DENV2(16.9%). DENV4 was not detected in any of the samples. The average Ct-value of all serotypes was 17.6, with DENV3 having the lowest Ct-value of 16.6, indicating slightly higher viremia. Conclusion Our study, although limited in its coverage of Nepal, has provided molecular information on the serotypes responsible for the 2022 dengue outbreak. The high prevalence of DENV1 was observed, while prevalence of DENV3 was accompanied by high viral load. KEY WORDS Dengue virus, Molecular, Serotypes, Nepal
dc.identifier.urihttps://hdl.handle.net/20.500.14572/4148
dc.language.isoen_US
dc.publisherKathmandu University
dc.subjectDengue virus
dc.subjectMolecular
dc.subjectSerotypes
dc.subjectNepal
dc.titleMolecular Investigation of Dengue Virus Serotypes in the Dengue Outbreak of 2022 in Nepal
dc.typeArticle
dspace.entity.typePublication
local.article.typeOriginal Article
oaire.citation.endPage106
oaire.citation.startPage99
relation.isJournalIssueOfPublication5a0c1a45-42a4-4aaf-9ef8-7a7b58ab2063
relation.isJournalIssueOfPublication.latestForDiscovery5a0c1a45-42a4-4aaf-9ef8-7a7b58ab2063
relation.isJournalOfPublicationa782b7ff-cf89-4178-ad1c-11ed89cfe1bd

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