Article: Antibiotic susceptibility pattern of pseudomonas aeruginosa and detection of virulence genes in a Tertiary care hospital, Kathmandu, Nepal
| creativeworkseries.issn | Online ISSN: 2676-1424; Print ISSN: 2676-1319 | |
| dc.contributor.author | Ojha, Tek Raj | |
| dc.contributor.author | Sah, Anil Kumar | |
| dc.contributor.author | Karn, Govind Lal | |
| dc.contributor.author | Parajuli, Pramila | |
| dc.creator.affiliation | Department of Microbiology, St. Xavier’s College, Maitighar, Kathmandu | |
| dc.creator.affiliation | Annapurna Neurological Institute and Allied Sciences, Maitighar, Kathmandu | |
| dc.creator.affiliation | Department of Microbiology, St. Xavier’s College, Maitighar, Kathmandu | |
| dc.creator.affiliation | Department of Microbiology, St. Xavier’s College, Maitighar, Kathmandu | |
| dc.date.accessioned | 2026-10-13T08:03:19Z | |
| dc.date.available | 2026-10-13T08:03:19Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Abstract: Pseudomonas aeruginosa is one of the most common causes of hospital-acquired infections. It threatens worldwide public health because of its intrinsic antibiotic resistance and virulence. In this context, this research aimed to determine the occurrence of P. aeruginosa in a clinical setting, examine its susceptibility to antibiotics, and detect the presence of virulence genes, viz. exoY, oprL and toxA in all isolates. It was a cross-sectional study carried out from June 2022 to December 2022. A total of 1356 clinical specimens were collected and processed in the laboratory for Gram staining, culture techniques, and biochemical tests. The Kirby-Bauer disk diffusion method was applied to examine the antibiotic susceptibility pattern and conventional PCR was used to detect the virulence genes in all confirmed P. aeruginosa isolates. 40.5% of specimens showed bacterial growth, of which only 3.02% were identified as P. aeruginosa. Among them, 61.0% were multidrug resistant and 29.2% were β-lactamase producers. Aztreonam (70.7%) was the most effective antibiotic. Among all P. aeruginosa isolates, 68.3% isolates were exoY positive, 61.0% were oprL positive and 56.1% were toxA positive. Strategic interventions are required to stop the emergence and spread of antimicrobial resistance as a result of the isolation of multidrug resistant (MDR) isolates carrying these virulence genes. This study can benefit the medical staff and the entire community in building a surveillance system and enhancing infection control procedures. | |
| dc.identifier.doi | https://doi.org/10.3126/nmcj.v26i3.69878 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14572/8160 | |
| dc.language.iso | en_US | |
| dc.publisher | Nepal Medical College Pvt. Ltd. | |
| dc.subject | Pseudomonas aeruginosa | |
| dc.subject | Antimicrobial resistance | |
| dc.subject | MDR | |
| dc.subject | β-lactamase | |
| dc.subject | Virulence genes | |
| dc.title | Antibiotic susceptibility pattern of pseudomonas aeruginosa and detection of virulence genes in a Tertiary care hospital, Kathmandu, Nepal | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.article.type | Original Article | |
| oaire.citation.endPage | 197 | |
| oaire.citation.startPage | 188 | |
| relation.isJournalIssueOfPublication | baa3fbfb-6e39-4595-a694-8c149ce4ad6a | |
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