Publication: A Comparative Study of Rapid SARS-Cov-2 Antigen Detection Assay against RT-PCR Assay for Diagnosis of COVID-19 in a Tertiary Hospital of Kathmandu
| creativeworkseries.issn | 1812-2027 | |
| dc.contributor.author | Chaudhary, R | |
| dc.contributor.author | Bhatta, S | |
| dc.contributor.author | Singh, A | |
| dc.contributor.author | Pradhan, M | |
| dc.contributor.author | Moktan, B | |
| dc.contributor.author | Duwal, S | |
| dc.contributor.author | Pandit, R | |
| dc.date.accessioned | 2025-12-26T08:22:11Z | |
| dc.date.available | 2025-12-26T08:22:11Z | |
| dc.date.issued | 2022 | |
| dc.description | Chaudhary R,1 Bhatta S,1 Singh A,1 Pradhan M,1 Moktan B,2 Duwal S,3 Pandit R4,5 1Department of Microbiology Nepalese Army Institute of Health Sciences(NAIHS) Bhandharkhal, Kathmandu, Nepal 2Department of Laboratory Medicine Shree Birendra Army Hospital Kathmandu, Nepal 3Department of Hygiene and Sanitation Shree Birendra Army Hospital Kathmandu, Nepal 4National Public Health Laboratory Department of Health Services Ministry of Health and Population Kathmandu, Nepal 5Shanghai Institute of Immunology Shanghai Jiao Tong University School of Medicine Shanghai, China | |
| dc.description.abstract | ABSTRACT Background The Coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus (SARS-CoV-2) has spread worldwide since its first recorded case in the city of Wuhan, China, in December 2019. SARS-CoV-2 infection causes asymptomatic to sever pneumonia. Severe cases may develop acute respiratory disease symdrome (ARDS), with an average mortality rate of 6.9%. Real Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR) assay is the current reference standard laboratory method for the diagnosis of SARS-CoV-2 infection. However, it takes around 6-8 hours to get the result and is time consuming. Therefore, rapid and accurate tests for SARS-CoV-2 screening are essential to expedite disease prevention and control. Lateral flow immunoassay using monoclonal anti SARS-CoV-2 antibodies which target for SARS-CoV-2 antigen can be complimentary screening test if their accuracy were comparable to that of the real time reverse transcription- polymerase chain reaction (RT-PCR) assay. Objective To find the sensitivity and specificity of a rapid antigentest kit in comparison to reverse transcription-polymerase chain reaction (RT-PCR). Method A cross-sectional hospital based study was carried out at Shree Birendra Army Hospital, Kathmandu for a period of four months. Result Our finding shows sensitivity and specificity of rapid diagnostic tests (RDT) Ag kit as 60.6% and 96.4% respectively. Positive and negative predictive value was 83.7% and 89.0%. Likewise, positive and negative likelihood ratio was 17.0 and 0.4. The overall accuracy of the antigen kit was 88.1% in comparison to reverse transcription- polymerase chain reaction (RT-PCR) as the gold standard. Conclusion Our study concluded the use of rapid antigen kit is mainly useful for screening purposes. KEY WORDS RDT Ag Kit, RT-PCR, SARS-CoV-2 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14572/3878 | |
| dc.language.iso | en_US | |
| dc.publisher | Kathmandu University | |
| dc.subject | RDT Ag Kit | |
| dc.subject | RT-PCR | |
| dc.subject | SARS-CoV-2 | |
| dc.title | A Comparative Study of Rapid SARS-Cov-2 Antigen Detection Assay against RT-PCR Assay for Diagnosis of COVID-19 in a Tertiary Hospital of Kathmandu | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.article.type | Original Article | |
| oaire.citation.endPage | 341 | |
| oaire.citation.startPage | 337 | |
| relation.isJournalIssueOfPublication | 70955ae5-defd-4f34-89b2-a5dc24ab2d4d | |
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