Article: Organism isolation in corneal ulcer- utility of different techniques
| creativeworkseries.issn | JNMA Print ISSN: 0028-2715; Online ISSN: 1815-672X | |
| dc.contributor.author | Thapa, M | |
| dc.contributor.author | Sharma, AK | |
| dc.contributor.author | Shah, DN | |
| dc.contributor.author | KC, S | |
| dc.contributor.author | Shrestha, S | |
| dc.creator.affiliation | B.P Koirala Lions Center for Ophthalmic studies, Institute of Medicine, Maharajgunj, Kathmandu. | |
| dc.creator.affiliation | B.P Koirala Lions Center for Ophthalmic studies, Institute of Medicine, Maharajgunj, Kathmandu. | |
| dc.creator.affiliation | B.P Koirala Lions Center for Ophthalmic studies, Institute of Medicine, Maharajgunj, Kathmandu. | |
| dc.creator.affiliation | Lumbini Eye Institute, Bhairahawa. | |
| dc.creator.affiliation | Lumbini Eye Institute, Bhairahawa. | |
| dc.date.accessioned | 2026-11-05T05:07:28Z | |
| dc.date.available | 2026-11-05T05:07:28Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | Abstract Introduction: Various techniques are available for isolation of microorganism in cases of microbial keratitis including conventional methods of scraping, re-scraping inoculation in different media and corneal biopsy. Methods: This was a cross sectional descriptive study conducted from February 2002 to July 2003. A total of 161 eyes of corneal ulcers were evaluated with scraping inoculation. Re-scraping and corneal biopsy were performed in those cases which did not grow any organism in the first scraping or which did not respond to initial treatment. Results: Microorganisms were isolated in 116 patients (72.04%) after first scraping. S pneumoniae was seen in 50(42%), Fusarium in 21(18%) and Aspergillus in 16(14%) cases. Re-scraping was done in 22 culture negative and non improving cases of which 14 (63.63%) showed isolation of organisms. After re-scraping, Fusarium and Aspergillus were isolated in four patients each (18% each) followed by S. pneumoniae in 3 (14%), S. aureus in 2 (9%), and Penicillium in 1(5%) case.Corneal biopsy was done in 10 cases with isolation of microbial agents in 7 (70%) with Aspergillus and S. aureus in three each, and S. epidermidis in 1(10%). Conclusions: Apart from conventional scraping, other isolation techniques should be considered in the treatment of non-responding and culture negative cases of corneal ulcers. Compared to bacteria, fungi are difficult to isolate in the first scraping and requires more frequent repeat scraping and corneal biopsy. Keywords: Corneal biopsy, Corneal scraping, Corneal ulcer, Microbial keratitis, Organismal isolation. | |
| dc.identifier.doi | https://doi.org/10.31729/jnma.47 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14572/8326 | |
| dc.language.iso | en_US | |
| dc.publisher | Nepal Medical Association | |
| dc.subject | Corneal biopsy | |
| dc.subject | Corneal scraping | |
| dc.subject | Corneal ulcer | |
| dc.subject | Microbial keratitis | |
| dc.subject | Organismal isolation | |
| dc.title | Organism isolation in corneal ulcer- utility of different techniques | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.article.type | Original Article | |
| oaire.citation.endPage | 19 | |
| oaire.citation.startPage | 14 | |
| relation.isJournalIssueOfPublication | 73dbc596-d145-4579-8718-17fd9ff19c7b | |
| relation.isJournalIssueOfPublication.latestForDiscovery | 73dbc596-d145-4579-8718-17fd9ff19c7b | |
| relation.isJournalOfPublication | e6e146a0-0ece-4aba-aa0a-6ccfbd10a12a |