Publication: A study of oxidative stress, antioxidant status and lipid profile in diabetic patient in the western region of Nepal
Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
Kathmandu University
Abstract
Abstract
Aims and Objectives: Diabetes mellitus (DM) is often termed as a disease of premature aging. Several studies have
indicated lopsided redox balance due to pro oxidant environment as one of the important etiological factors. Some
recent researches also indicate a causal relationship with oxidative stress (OS). So far, no study has been undertaken
on this aspect in Nepali populations. We, therefore, aimed this maiden study in Nepali population to examine redox
balance by measuring OS and antioxidant status along with lipid profile in 37 patients of DM type- 2 and 30
matched normal subjects.
Methodology: Thirty seven patients of DM type-2 without any complications (mean age= 57.6± 10.6 years) and 30
normal subjects (mean age= 55.8 ± 14.8 years) were included in this study. Body Mass Index (BMI) and Waist/Hip
(W/H) ratio were measured. Fasting blood sample was collected for the analysis of total antioxidant activity (TAA),
plasma and urinary thiobarbituric acid reactive substances (TBARS) and lipid profile by standard procedures in both
the groups. The statistical analysis was done with SPSS 10 version.
Results: Total cholesterol, triglyceride, VLDL-cholesterol, LDL-cholesterol, plasma and urinary TBARS were
significantly raised whereas, plasma TAA was significantly reduced in DM type-2 patients as compared to controls.
The comparison of old and fresh cases revealed that though TAA was lower and PTBARS and UTBARS were
higher in patients but did not attain the level of significance. W/H ratio is significantly higher in patients compared
to normal subjects. But, no significant correlation of BMI and W/H with lipid profile is observed in both control and
patients.
Conclusion: Oxidative stress is raised in type 2 DM patients. This along with deranged lipid profile and decreased
antioxidant status could be the risk factors in the development of complications associated with DM.
Key words: oxidative stress, antioxidant, lipid profile, diabetes mellitus, Nepal.
Description
Maharjan BR1, Jha JC2, Adhikari D2, Vishwanath P2, Baxi J3, Alurkar VM 4, Singh PP5
1Lecturer, Department of Biochemistry, Kathmandu University of Medical Sciences, Kavre, Nepal, 2Lecturer, 5Professor, Department of Biochemistry, 3Associate Professor, Department of Surgery, 4Professor, Department of Medicine, Manipal College of Medical Sciences, Pokhara, Nepal