Publication: Rotavirus and its Genotype Distribution among Children Less than Three Years Presenting with Acute Watery Diarrhoea to a General Hospital in Urban Nepal
Date
2011
Article Type
Original Article
Journal Title
Journal ISSN
ISSN 1990-7974 eISSN 1990-7982
Volume Title
Pages
Pages: 110 - 115
Publisher
Nepal Paediatric Society (JNPS)
Abstract
Abstract:
Introduction: Viruses are the most common cause for diarrhoea in infants and small children. Rotavirus is the most frequent viral etiology, causing 125 million episodes of infantile diarrhoea and over 600,000 deaths per year.
Materials and methods: A cross sectional study between January and March 2008 was conducted at Patan Hospital, Kathmandu to find out the prevalence of rotavirus among children < 3 years with watery diarrhoea and to identify common strains of rotavirus in the study population. Testing for rotavirus was undertaken by using Rota/Adeno screen Dipstick M583CE. Rotavirus strain identification was done at Nagasaki University Japan. Data was analyzed using SPSSĀ® for Windows V 15.0 software.
Results: 119 children with acute watery diarrhoea were enrolled. Rotavirus antigen was found in 63 cases (53%). The highest percentage of rotavirus infection was found in the second six months of life. Among the children with positive rotavirus antigen, the median age was 10 months (IRQ 8.00). The most predominant strain of rotavirus identified was G12 followed by G9 and G1. Most predominant G and P combination was G9 P[8] followed by G12P[6].
Conclusion: The study has shown the prevalence of unusual serotypes of rotavirus. Though rotavirus vaccine has been studied, used widely and found to be very effective, none of the vaccine efficacy studies have included common serotypes identified in Nepal. Level of protection conferred by infant immunization with the current rotavirus vaccines against the strains circulating in Nepal is unknown and careful surveillance through vaccine implementation is needed.
Description
Shrijana Shrestha
Professor Department of Paediatrics, Paediatric Research Unit, Department of Paediatrics, Patan Hospital, Patan Academy of Health Sciences, Kathmandu
Bishwas Upadhyay
Paediatric Research Unit, Department of Paediatrics, Patan Hospital, Patan Academy of Health Sciences, Kathmandu
Bhupendra Limbu
Paediatric Research Unit, Department of Paediatrics, Patan Hospital, Patan Academy of Health Sciences, Kathmandu
Rahul Pradhan
Paediatric Research Unit, Department of Paediatrics, Patan Hospital, Patan Academy of Health Sciences, Kathmandu
Tokoko Nakagomi
Division of Molecular Epidemiology, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University
Stephen Thorson
Paediatric Research Unit, Department of Paediatrics, Patan Hospital, Patan Academy of Health Sciences, Kathmandu
Andrew J Pollard
Oxford Vaccine Group, University Department of Paediatrics, University of Oxford, Oxford
Neelam Adhikari
Professor in Paediatrics. Paediatric Research Unit, Department of Paediatrics, Patan Hospital, Patan Academy of Health Sciences, Kathmandu
Keywords
Diarrhoea, Nepal, rotavirus, rotavirus genotypes, vaccine
Identifier
https://doi.org/10.3126/jnps.v31i2.4641