Publication: Phenotypic characterisation and drug sensitivity testing of mycobacteria isolated from extra-pulmonary tuberculosis
Date
2010
Journal Title
Journal ISSN
Volume Title
Publisher
Kathmandu University
Abstract
Abstract
Background: Tuberculosis (TB) is a major cause of morbidity and mortality globally, with most cases occurring in
developing countries. The importance of extra pulmonary tuberculosis (EPTB) among all forms of tuberculosis has not
yet been ascertained in developing countries
Objective: To identify and perform phenotypic characterization of clinically important mycobacterium isolates from
extra-pulmonary sites along with drug susceptibility testing.
Materials and methods: A total of 513 specimens from patients of (EPTB) with varied presentation were studied.
Speciation and characterization of isolates were done on the basis of growth and biochemical characteristics. Drug
susceptibility testing for mycobacterium isolates was done by proportion method.
Results: Fifty four patients clinically suspected to have extra-pulmonary tuberculosis were culture positive for
mycobacteria. On characterization 48 of the 54 isolates were identified as M. tuberculosis, 4 as M. bovis and 2 were M.
avium/ intracelulare.
Drug susceptibility testing was done for the both M. tuberculosis as well as M. bovis. In single drug resistance,
Streptomycin was highest followed by Isoniazid, Ethambutol and Refampicin. Multi drug resistance (MDR) was found
in 6 isolates and it was observed only in group I cases.
Conclusion: EPTB accounts for 10-15 percent of all cases of tuberculosis. Mycobacterium was present in 10.5 percent
samples. 48 isolates out of 54 samples were found to be M. tuberculosis. The maximum numbers of M. tuberculosis were
isolated from lymph node aspiration.
Key words: Phenotypic characterization, drug susceptibility, extra pulmonary tuberculosis
Description
Gurung R 1, Bhattacharya SK1 , Pradhan B 1, Gurung S 1, Singh YI 2
1 B. P. Koirala Institute of Health Sciences, Dharan, Nepal. 2Manipal College of Medical Sciences, Pokhara, Nepal