Publication: Persistent Positivity of Reverse Transcriptase-Polymerase Chain Reaction Test among Patients with COVID-19 in Rural Teaching Hospital: A Descriptive Cross-sectional Study
| creativeworkseries.issn | JNMA Print ISSN: 0028-2715; Online ISSN: 1815-672X | |
| dc.contributor.author | Maharjan, Narayani | |
| dc.contributor.author | Thapa, Niresh | |
| dc.contributor.author | Magar, Bibek Pun | |
| dc.contributor.author | Maharjan, Muna | |
| dc.contributor.author | Tu, Jiancheng | |
| dc.date.accessioned | 2026-02-12T08:38:48Z | |
| dc.date.available | 2026-02-12T08:38:48Z | |
| dc.date.issued | 2021 | |
| dc.description | Narayani Maharjan Program & Department of Clinical Laboratory Medicine, Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China and Department of Molecular Laboratory, Karnali Academy of Health Sciences, Jumla, Nepal http://orcid.org/0000-0002-8930-7409 Niresh Thapa Department of General Practice and Emergency Medicine, Karnali Academy of Health Sciences, Jumla, Nepal https://orcid.org/0000-0003-3641-5622 Bibek Pun Magar Department of Clinical Biochemistry, Karnali Academy of Health Sciences, Jumla, Nepal Muna Maharjan School of Nursing and Midwifery, Karnali Academy of Health Sciences, Jumla, Nepal https://orcid.org/0000-0001-8601-8620 Jiancheng Tu Program & Department of Clinical Laboratory Medicine, Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China https://orcid.org/0000-0003-4304-1593 | |
| dc.description.abstract | Abstract: Introduction: The persistence positivity detected for severe acute respiratory syndrome coronavirus 2 ribonucleic acid by real-time reverse transcriptase-polymerase chain reaction test in asymptomatic coronavirus disease 2019 positive patients has attracted a lot of attention. There is limited data on the duration of viral shedding. We aimed to determine the proportion of coronavirus disease patients with persistent positivity of real-time reverse transcriptase-polymerase chain reaction test in a teaching hospital of Nepal. Methods: A descriptive cross-sectional study was conducted using medical records from May to September 2020 in a teaching hospital of Nepal. The study was approved by the Institutional Review Committee of Karnali Academy of Health Sciences (Reference no 077/078/03). Convenient sampling method was used. Data was analysed by Statistical Package for the Social Sciences. Point estimate at 90% Confidence Interval was calculated along with frequency and proportion for binary data. Results: Of the total 95 cases, 9 (9.5%) cases (4.6-14.4 at 90% Confidence Interval), were repeat positive after achieving the first negative. The mean day required of achieving the last negative for the repeat positive group was 62.11±3.95, range (60-70 days). The mean time duration for the virus shedding was found to be 20.43±12.19 days (range 7-60 days) after the first positive test result. Conclusions: This study concludes that there might be a persistent positivity of the polymerase chain reaction test among patients with COVID-19. The majority of the patients were test positive for 8-14 days, and some were positive till 60-70 days. | |
| dc.identifier | https://doi.org/10.31729/jnma.7077 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14572/4729 | |
| dc.language.iso | en_US | |
| dc.publisher | Nepal Medical Association | |
| dc.subject | COVID-19 | |
| dc.subject | recurrence | |
| dc.subject | reverse transcriptase-polymerase chain reaction | |
| dc.subject | SARS-CoV-2 | |
| dc.subject | viral shedding. | |
| dc.title | Persistent Positivity of Reverse Transcriptase-Polymerase Chain Reaction Test among Patients with COVID-19 in Rural Teaching Hospital: A Descriptive Cross-sectional Study | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.article.type | Original Article | |
| oaire.citation.endPage | 1140 | |
| oaire.citation.startPage | 1136 | |
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