Publication: Phenotypic and Genotypic Detection of Metallo-b Lactamase in Non-Fermenting Gram Negative Bacilli Obtained from Clinical Samples in a Tertiary Care Hospital in Nepal
| creativeworkseries.issn | ISSN (Print) : 1993-2979 | ISSN (Online) : 1993-2987 | |
| dc.contributor.author | Sapkota, Jyotshna | |
| dc.contributor.author | GC, Divya Shree | |
| dc.contributor.author | Adhikari, Ram Prasad | |
| dc.contributor.author | Khadka, Shusila | |
| dc.contributor.author | Pandey, Ritu | |
| dc.contributor.author | Sah, Anil Kumar | |
| dc.contributor.author | Khanal, Laxmi Kant | |
| dc.date.accessioned | 2025-11-04T04:47:41Z | |
| dc.date.available | 2025-11-04T04:47:41Z | |
| dc.date.issued | 2025 | |
| dc.description | Jyotshna Sapkota Department of Microbiology, Nepal Medical College Teaching Hospital, Kathmandu, Nepal Author Divya Shree GC Department of Microbiology, Nepal Medical College Teaching Hospital, Kathmandu, Nepal Author Ram Prasad Adhikari Department of Microbiology, Nepal Medical College Teaching Hospital, Kathmandu, Nepal Author Shusila Khadka Department of Microbiology, Nepal Medical College Teaching Hospital, Kathmandu, Nepal Author Ritu Pandey Department of Microbiology, Nepal Medical College Teaching Hospital, Kathmandu, Nepal Author Anil Kumar Sah Department of Microbiology, Nepal Medical College Teaching Hospital, Kathmandu, Nepal Author Laxmi Kant Khanal Department of Microbiology, Nepal Medical College Teaching Hospital, Kathmandu, Nepal Author | |
| dc.description.abstract | Abstract: Introduction Non-fermenting Gram-negative bacilli, including Pseudomonas aeruginosa and Acinetobacter baumannii, are significant nosocomial pathogens with limited treatment options due to their intrinsic and acquired resistance mechanisms. Among these, metallo-β-lactamases are of major concern which hydrolyze carbapenems and contribute to antimicrobial resistance. This study aimed to determine the prevalence of Metallo-β-Lactamase-producing non-fermenting gram negative bacilli in clinical specimens using both phenotypic and genotypic methods. Methods A descriptive cross-sectional study was conducted at Nepal Medical College Teaching Hospital from January 2024 to December 2024. A total of 16,954 clinical specimens were processed for culture and sensitivity testing. NFGNB were identified using standard microbiological methods. Antimicrobial susceptibility testing was conducted using the Kirby-Bauer disk diffusion method following Clinical and Laboratory Standards Institute guidelines. MBL production was detected phenotypically using the Imipenem-EDTA combined disc method. The presence of IMP and VIM genes was confirmed by conventional PCR. Results Among 16,954 specimens, 163 (0.96%) NFGNB isolates were identified, with Pseudomonas aeruginosa (52.1%) being the most prevalent, followed by Acinetobacter species (39.3%) and Burkholderia species (8.6%). MBL production was detected in 22 (13.5%) isolates: Pseudomonas aeruginosa (59.1%), and Acinetobacter species (40.9%). Genotypically IMP and VIM genes were found in 36.4% and 31.8% of MBL-positive isolates, respectively, while one isolate harbored both genes. Notably, 27.3% of phenotypic MBL producers tested negative for both IMP and VIM, suggesting the potential involvement of other MBL genes. Conclusion The significant prevalence of MBL-producing NLFGNB, particularly among Pseudomonas aeruginosa and Acinetobacter species, highlights a serious challenge for antimicrobial therapy and underscores an urgent need for robust infection control and antimicrobial stewardship strategies. | |
| dc.identifier | https://doi.org/10.59779/jiomnepal.1383 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14572/3030 | |
| dc.language.iso | en_US | |
| dc.publisher | Institute of Medicine, Tribhuvan University | |
| dc.subject | Metallo-β-lactamase | |
| dc.subject | Carbapenem resistance | |
| dc.subject | Non-fermenting Gram-negative bacilli | |
| dc.subject | Acinetobacter species | |
| dc.subject | Pseudomonas aeruginosa | |
| dc.subject | IMP gene | |
| dc.subject | VIM gene | |
| dc.title | Phenotypic and Genotypic Detection of Metallo-b Lactamase in Non-Fermenting Gram Negative Bacilli Obtained from Clinical Samples in a Tertiary Care Hospital in Nepal | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.article.type | Original Article | |
| oaire.citation.endPage | 113 | |
| oaire.citation.startPage | 108 | |
| relation.isJournalIssueOfPublication | 36785908-a57b-42f4-961a-9168ac067265 | |
| relation.isJournalIssueOfPublication.latestForDiscovery | 36785908-a57b-42f4-961a-9168ac067265 | |
| relation.isJournalOfPublication | a9ba45d9-ee33-4a6b-b1fc-6626b87eec6c |