Publication:
Prevalence of COVID-19 Positive Cases Diagnosed by Real time Polymerase Chain Reaction in a Tertiary Care Hospital of Nepal

Simple item page
creativeworkseries.issnJNMA Print ISSN: 0028-2715; Online ISSN: 1815-672X
dc.contributor.authorPokharel, Khilasa
dc.contributor.authorMishra, Bhavesh
dc.contributor.authorKarki, Anup
dc.date.accessioned2026-03-13T05:16:57Z
dc.date.available2026-03-13T05:16:57Z
dc.date.issued2021
dc.descriptionKhilasa Pokharel Department of Microbiology, Kathmandu Medical College and Teaching Hospital, Kathmandu, Nepal Bhavesh Mishra Department of Microbiology, Kathmandu Medical College and Teaching Hospital, Kathmandu, Nepal Anup Karki Department of Microbiology, Kathmandu Medical College and Teaching Hospital, Kathmandu, Nepal
dc.description.abstractAbstract: Introduction: The virus that causes COVID-19 is known as severe acute respiratory syndrome Coronavirus-2. This new variant of Corona Virus introduced in China has urged the massive health system resources to focus on its screening and management of sick patients worldwide. We aimed to find the prevalence of COVID-19 positive cases diagnosed by Real-time polymerase chain reaction in a tertiary care hospital of Nepal. Methods: This is a descriptive cross-sectional study that was conducted from 11th of November to 15th December 2020. Nasopharyngeal and Oropharyngeal swabs were collected, and confirmation of cases of COVID-19 was done based on the detection of viral ribonucleic acid by nucleic acid amplification tests such as real-time reverse transcriptase-polymerase chain reactions. The viral genes targeted include the E, N, and ORF. Results: A total of 15247 samples have been processed, of which s (14.81%) positive cases were included in this study. There were 1427 (63.19%) male and 831 (36.68%) females. The majority of the cases were asymptomatic 1386 (61.38%). The most common age group infected was between 15 to 40 years, 841 (58.93%) male and 542 (65.22%) females. The most common presenting symptoms were cough 315 (13.95%) and fever 306 (13.55%). Conclusions: Most of the individuals reported for real-time polymerase chain reaction were asymptomatic patients who might be contagious and have the potential to transmit infection. Among symptomatic cases, common symptoms were cough and fever.
dc.identifierhttps://doi.org/10.31729/jnma.5992
dc.identifier.urihttps://hdl.handle.net/20.500.14572/5093
dc.language.isoen_US
dc.publisherNepal Medical Association
dc.subjectasymptomatic
dc.subjectCOVID-19
dc.subjectcycle threshold value
dc.subjectreverse transcriptase polymerase chain reaction
dc.titlePrevalence of COVID-19 Positive Cases Diagnosed by Real time Polymerase Chain Reaction in a Tertiary Care Hospital of Nepal
dc.typeArticle
dspace.entity.typePublication
local.article.typeOriginal Article
oaire.citation.endPage251
oaire.citation.startPage248
relation.isJournalIssueOfPublication7e76f436-e390-4a91-a8f7-b1d169ad359c
relation.isJournalIssueOfPublication.latestForDiscovery7e76f436-e390-4a91-a8f7-b1d169ad359c
relation.isJournalOfPublicatione6e146a0-0ece-4aba-aa0a-6ccfbd10a12a

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
248-251.pdf
Size:
290.35 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.86 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Publications

Connect with us

Nepal Health Research Council © 2026
Ramshah Path, Kathmandu Nepal P.O.Box 7626