Article:
Nepal’s First Whole Genome Sequencing of the Monkeypox Virus: Imported Cases from Saudi Arabia with Evidences of Secondary Transmission

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creativeworkseries.issn1999-6217
dc.contributor.authorJha, Shivendra Kumar
dc.contributor.authorKarna, Priya
dc.contributor.authorPandey, Gaurab
dc.contributor.authorKunwar, Pratik
dc.contributor.authorBhatta, Ranjan Raj
dc.contributor.authorBhusal, Nirajan
dc.date.accessioned2026-08-25T10:45:39Z
dc.date.available2026-08-25T10:45:39Z
dc.date.issued2025
dc.descriptionShivendra Kumar Jha Sukraraj Tropical and Infectious Diseases Hospital, Teku, Kathmandu, Nepal Priya Karna Sukraraj Tropical and Infectious Diseases Hospital, Teku, Kathmandu, Nepal Gaurab Pandey National Public Health Laboratory, Teku, Kathmandu, Nepal Pratik Kunwar National Public Health Laboratory, Teku, Kathmandu, Nepal Ranjan Raj Bhatta National Public Health Laboratory, Teku, Kathmandu, Nepal Nirajan Bhusal National Public Health Laboratory, Teku, Kathmandu, Nepal
dc.description.abstractAbstract: Monkeypox (Mpox) is an infection caused by a zoonotic orthopoxvirus that is being reported in non-endemic regions. We present here 4 confirmed and probable cases managed at Sukraraj Tropical and Infectious Disease Hospital, Teku, Kathmandu. Three patients were Nepalese men returning from Saudi Arabia with fever, malaise, and papulopustular rashes. The fourth case, the wife of one patient, developed fever, myalgia, lethargy, headache, and later vaginal pustular lesions progressing to ulcers, indicating secondary transmission. Lesion swabs and crust samples underwent real-time PCR at the National Public Health Laboratory (NPHL), Teku, Kathmandu, Nepal targeting G2R and F3L genes, all testing positive (Ct ?35). Positive samples were sequenced in-country at NPHL for the first time using the PrimalSeq protocol on an Illumina MiSeq platform. Genome analysis confirmed Clade IIb A.2, matching strains circulating in Europe and the Middle East. NPHL’s clade identification was concordant with WHO reference laboratories, validating Nepal’s genomic capacity.
dc.identifierhttps://doi.org/10.33314/jnhrc.v23i04.4941
dc.identifier.urihttps://hdl.handle.net/20.500.14572/7478
dc.language.isoen_US
dc.publisherNepal Health Research Council
dc.subjectClade IIb A.2
dc.subjectGenome sequencing
dc.subjectMonkeypox
dc.subjectMpox
dc.subjectNepal
dc.titleNepal’s First Whole Genome Sequencing of the Monkeypox Virus: Imported Cases from Saudi Arabia with Evidences of Secondary Transmission
dc.typeArticle
dspace.entity.typePublication
local.article.typeCase Report
oaire.citation.endPage811
oaire.citation.startPage809
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relation.isJournalOfPublication40bd2739-8b19-447c-be60-723a1bdd1dcd

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