Publication: Genetic variability of the human filarial parasite, Wuchereria bancrofti in Southern parts of Nepal
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Institute of Medicine
Abstract
Abstract
Introduction: Lymphatic filariasis (LF) is a neglected tropical disease, caused by Wuchereria bancrofti parasite and pose potential risk to about 1.4 billion people in 73 countries. In Nepal, 60 out of 75 districts are endemic and nearly 90% of the population is at risk and mass drug administration (MDA) has beenin place since 2003. Yet, information about the genetic diversity of W. bancrofti is largely lacking.
Methods: The genetic variability of the parasites in two localities Kailali and Kapilbastu from south- western parts of Nepal were studied. Blood samples were collected at night and stained with Giemsa stain and positive archived slides were taken for the study. W. Bancrofti was individually picked under microscope and gDNA was isolated from the pooled (100 mf) samples. Short Tandem 29bp Repeats (STR) from the intronic region of Abundant Larval Transcript-2 (ALT-2) gene and haplotype mapping of the Internal Transcribed Spacer (ITS-1) region were studied. The phylogenetic trees were constructed and analyzed.
Results: The analyses of STR, haplotypes and the phylogenetic trees indicated the presence of at least two genetically distinct clusters among the W. Bancrofti parasite populations in two areas.
Conclusion: The study identified two genetically distinct clusters of mf in the populations. The finding of two genetic “variants” of W. bancrofti in the present study has important implications for filariasis epidemiology and control/elimination program.
Keywords: Genetic variability, Lymphatic filariasis, Nepal, Wuchereria bancrofti
Description
RK Adhikari
Institute of Medicine, Maharajgunj Medical Campus, Tribhuvan University, Kathmandu, Nepal
JB Sherchand
Institute of Medicine, Maharajgunj Medical Campus, Tribhuvan University, Kathmandu, Nepal
SL Hoti
Regional Medical Research Centre, Indian Council for Medical Research, Department of Health research, Government of India, Nehru Nagar, Belgaum, Karnataka
LA Vishal
Regional Medical Research Centre, Indian Council for Medical Research, Department of Health research, Government of India, Nehru Nagar, Belgaum, Karnataka
SR Mishra
Manmohan Memorial Institute of Health Sciences, Nepal
EJW Weber
Fordham University, Bronx, New York, USA
PR Khanal
Manmohan Memorial Institute of Health Sciences, Nepal
G Joshi
Manmohan Memorial Institute of Health Sciences, Nepal
SP Dumre
World Health Organization, Country office, Nepa
R Adhikari
Manmohan Memorial Institute of Health Sciences, Nepal
S Nazeer
Regional Medical Research Centre, Indian Council for Medical Research, Department of Health research, Government of India, Nehru Nagar, Belgaum, Karnataka
K Ranabhat
National Public Health Laboratory, Kathmandu, Nepal
RR Wagle
Institute of Medicine, Maharajgunj Medical Campus, Kathmandu, Nepal
Keywords
Genetic variability, Lymphatic filariasis, Nepal, Wuchereria bancrofti