Publication: Ampicillinase C Beta-lactamase Producers among Isolates of Enterobacteriaceae in a Tertiary Care Centre: A Descriptive Cross-sectional Study
| creativeworkseries.issn | JNMA Print ISSN: 0028-2715; Online ISSN: 1815-672X | |
| dc.contributor.author | Khadka, Shusila | |
| dc.contributor.author | Barakoti, Achut | |
| dc.contributor.author | Adhikari, Ram Prasad | |
| dc.contributor.author | Khanal, Laxmi Kant | |
| dc.contributor.author | Sapkota, Jyotshna | |
| dc.date.accessioned | 2025-12-01T08:58:47Z | |
| dc.date.available | 2025-12-01T08:58:47Z | |
| dc.date.issued | 2022 | |
| dc.description | Shusila Khadka Department of Microbiology, Nepal Medical College and Teaching Hospital, Jorpati, Kathmandu Nepal Achut Barakoti Department of Microbiology, Nepal Medical College and Teaching Hospital, Jorpati, Kathmandu Nepal Ram Prasad Adhikari Department of Microbiology, Nepal Medical College and Teaching Hospital, Jorpati, Kathmandu Nepal Laxmi Kant Khanal Department of Microbiology, Nepal Medical College and Teaching Hospital, Jorpati, Kathmandu Nepal Jyotshna Sapkota Department of Microbiology, Nepal Medical College and Teaching Hospital, Jorpati, Kathmandu Nepal | |
| dc.description.abstract | Abstract Introduction: Ampicillinase C beta-lactamase-producing organisms are often resistant to multiple antimicrobial agents, and therapeutic options against these pathogens are limited. Limited information is available regarding Ampicillinase C beta-lactamase producers. The aim of this study was to find out the prevalence of Ampicillinase C beta-lactamase producers among isolates of Enterobacteriaceae in a tertiary care centre. Methods: A descriptive cross-sectional study was carried out in the Clinical Microbiology Laboratory of a tertiary care centre from May 2021 to October 2021. Ethical approval was received from the Institutional Review Committee (Reference number: 044-077/078). Isolates of Enterobacteriaceae from various clinical samples were collected by convenience sampling. Ampicillinase C screening for beta-lactamase producers among the Enterobacteriaceae isolates was done using cefoxitin (30 μg) disc. Detection of Ampicillinase C beta-lactamase producers among the screen-positive isolates was done by cefoxitin-cloxacillin double-disc synergy test. An increase in the zone size of ≥4 mm was considered as Ampicillinase C beta-lactamase producers. Point estimate and 95% Confidence Interval were calculated. Results: Among the total 481 isolates of Enterobacteriaceae, 49 (10.19%) (7.50-12.90, 95 % Confidence Interval) were detected as Ampicillinase C beta-lactamase producers among isolates of Enterobacteriaceae. Conclusions: The prevalence of Ampicillinase C beta-lactamase producers was lower than in other studies done in similar settings. Meropenem could be a drug of choice for the treatment of infections due to Ampicillinase C beta-lactamase-producing gram-negative bacteria. | |
| dc.identifier | https://doi.org/10.31729/jnma.7536 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14572/3345 | |
| dc.language.iso | en_US | |
| dc.publisher | Nepal Medical Association | |
| dc.subject | Antibiotic | |
| dc.subject | Beta-lactamase | |
| dc.subject | Enterobacteriaceae | |
| dc.subject | Gram-negative bacteria | |
| dc.title | Ampicillinase C Beta-lactamase Producers among Isolates of Enterobacteriaceae in a Tertiary Care Centre: A Descriptive Cross-sectional Study | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.article.type | Original Article | |
| oaire.citation.endPage | 692 | |
| oaire.citation.startPage | 689 | |
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