Publication: Microbiological Analysis of the Urine Isolates in Kathmandu Medical College Teaching Hospital, Kathmandu, Nepal
Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
Kathmandu University
Abstract
ABSTRACT
Background
Urinary tract infections (UTIs) are the common cause of bacterial infection. Recently
UTI become more complicated and difficult to treat because of appearance of
pathogen with increasing resistance to antimicrobial agents.
Objective
To determine the etiology of the urinary tract infections and their susceptibility to
antimicrobial agents.
Methods
This study was carried out in Kathmandu Medical College, at department of
microbiology. Total 3,460 urine samples were tested microbiologically by standard
procedure. Antibiotic susceptibility test was performed for all the isolates by Kirby
Bauer disc diffusion method and result was interpreted according to National
Committee for Clinical Laboratory Standards (NCCLS) guide line.
Results
Out of 3,460 urine samples 680 (19.7%) showed the significant bacteriuria. The
most common pathogens isolated were Escherichia coli 75.7% followed by Klebsiella
pneumoniae 10.7%, Acinetobacter spp 5.5%, Proteus spp 3.5% and Pseudomonas
aeruginosa 1.2%. Most susceptible antibiotic was Amikacin, Ceftriaxone and
Ciprofloxacin for most of the isolates. E. coli which was the main isolate was found
to be most susceptible to Amikacin 96.1%, Nitrofurantoin 91.3% and Gentamicin
77.7% followed by Ceftriaxone 65.8% and Ciprofloxacin 64.1%.
Conclusion
Regular surveillance of the resistance rate among uro-pathogens is needed to
ensure the appropriate therapy of UTI.
KEY WORDS
antibiotic susceptibility, bacteriuria, UTI.
Description
Raza S, Pandey S, Bhatt CP
Department of Microbiology Kathmandu Medical College Teaching Hospital Sinamangal, Kathmandu, Nepal