Publication:
Molecular epidemiology of Rotavirus diarrhea among children in Nepal: Emergence of G12 and G9 strains

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creativeworkseries.issnISSN (Print) : 1993-2979 | ISSN (Online) : 1993-2987
dc.contributor.authorSherchand, JB
dc.contributor.authorTandukar, S
dc.contributor.authorSherchan, JB
dc.contributor.authorGurung, S
dc.contributor.authorDhakwa, JR
dc.contributor.authorBichha, RP
dc.contributor.authorMahaseth, C
dc.date.accessioned2026-05-18T05:59:19Z
dc.date.available2026-05-18T05:59:19Z
dc.date.issued2013
dc.descriptionJB Sherchand Tribhuvan University Institute of Medicine, Public Health Research Laboratory and Microbiology S Tandukar Tribhuvan University Institute of Medicine, Public Health Research Laboratory and Microbiology JB Sherchan Department of Microbiology, Kathmandu University School of Medical Sciences, Dhulikhel S Gurung Program for Immunization Preventable Diseases (IPD), Kathmandu, Nepal JR Dhakwa Kanti Children’s Hospital, Maharajgunj, Kathmandu, Nepal RP Bichha Kanti Children’s Hospital, Maharajgunj, Kathmandu, Nepal C Mahaseth Kanti Children’s Hospital, Maharajgunj, Kathmandu, Nepal
dc.description.abstractAbstract Introduction: Rotavirus is the leading cause of diarrhea and dehydration among infants in both developed and developing countries. The primary objective of this study was to find the magnitude of rotavirus disease burden and genotypic variations of rotavirus. Methodology: Questionnaires and stool samples were collected from 1003 enrolled children under 5 years of age attending tertiary care Children’s Hospital with acute watery diarrhea during January to December 2012. Rotavirus in stool samples was detected by Enzyme Immuno Assay (EIA) and strains detected from rotavirus positive samples were genotyped by Reverse-Transcription Polymerase Chain reaction (RT-PCR). Results: Among these, 356 (35.4%) cases were positive for rotavirus by EIA, among the positive cases, 344 samples underwent genotyping by RT-PCR. Rotavirus positive cases were predominant in children who were admitted to the hospital which was 37.8% (115 out of 336). Overall G12 was the most prevalent genotype (52.3%), followed by G1 (17.7%), G2 (10.17%) and G9 (8.1%). The P types identified were P[6] (55.23%), P[8] (20%), and P[4] (12.5%). Conclusion: The study reveals that rotavirus gastroenteritis accounted for more than one-third of all cases of acute diarrhea. Use of rotavirus vaccines may reduce of high burden of rotavirus diarrhea in children. Emergence of G12 and G9 strains proves the immediate need of vaccine in Nepal. Keywords: Molecular epidemiology, rotavirus, Nepal
dc.identifierhttps://doi.org/10.59779/jiomnepal.591
dc.identifier.urihttps://hdl.handle.net/20.500.14572/6105
dc.language.isoen_US
dc.publisherInstitute of Medicine
dc.subjectMolecular epidemiology
dc.subjectrotavirus
dc.subjectnepal
dc.titleMolecular epidemiology of Rotavirus diarrhea among children in Nepal: Emergence of G12 and G9 strains
dc.typeArticle
dspace.entity.typePublication
local.article.typeOriginal Article
oaire.citation.endPage10
oaire.citation.startPage3
relation.isJournalIssueOfPublication4c7059ac-900c-4673-bc59-61e668eda4bc
relation.isJournalIssueOfPublication.latestForDiscovery4c7059ac-900c-4673-bc59-61e668eda4bc
relation.isJournalOfPublicationa9ba45d9-ee33-4a6b-b1fc-6626b87eec6c

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