Browsing by Author "Alurkar, VM"
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Publication A study of oxidative stress, antioxidant status and lipid profile in diabetic patient in the western region of Nepal(Kathmandu University, 2008) Maharjan, BR; Jha, JC; Adhikari, D; Vishwanath, P; Baxi, J; Alurkar, VM; Singh, PPAbstract Aims and Objectives: Diabetes mellitus (DM) is often termed as a disease of premature aging. Several studies have indicated lopsided redox balance due to pro oxidant environment as one of the important etiological factors. Some recent researches also indicate a causal relationship with oxidative stress (OS). So far, no study has been undertaken on this aspect in Nepali populations. We, therefore, aimed this maiden study in Nepali population to examine redox balance by measuring OS and antioxidant status along with lipid profile in 37 patients of DM type- 2 and 30 matched normal subjects. Methodology: Thirty seven patients of DM type-2 without any complications (mean age= 57.6± 10.6 years) and 30 normal subjects (mean age= 55.8 ± 14.8 years) were included in this study. Body Mass Index (BMI) and Waist/Hip (W/H) ratio were measured. Fasting blood sample was collected for the analysis of total antioxidant activity (TAA), plasma and urinary thiobarbituric acid reactive substances (TBARS) and lipid profile by standard procedures in both the groups. The statistical analysis was done with SPSS 10 version. Results: Total cholesterol, triglyceride, VLDL-cholesterol, LDL-cholesterol, plasma and urinary TBARS were significantly raised whereas, plasma TAA was significantly reduced in DM type-2 patients as compared to controls. The comparison of old and fresh cases revealed that though TAA was lower and PTBARS and UTBARS were higher in patients but did not attain the level of significance. W/H ratio is significantly higher in patients compared to normal subjects. But, no significant correlation of BMI and W/H with lipid profile is observed in both control and patients. Conclusion: Oxidative stress is raised in type 2 DM patients. This along with deranged lipid profile and decreased antioxidant status could be the risk factors in the development of complications associated with DM. Key words: oxidative stress, antioxidant, lipid profile, diabetes mellitus, Nepal.Publication Oxidative stress and antioxidant status in cardiovascular diseases in population of western Nepal(Kathmandu University, 2006) Risal, S; Adhikari, D; Alurkar, VM; Singh, PPObjectives: To observe if there is any connectivity between oxidative stress and cardiovascular diseases (CVDs). Materials and methods: Patients suffering from different cardiovascular diseases (hypertension, ischemic heart disease, rheumatic heart disease) attending Manipal Teaching Hospital, Pokhara and strictly matched controls were selected for this study. Oxidative stress (OS) was measured by plasma thiobarbituric acid reacting substances (TBARS) where as antioxidant status was measured by estimating vitamin E, vitamin C and total antioxidant activity (TAA) in plasma. Results: The mean level of TBARS, TAA, vitamin C and E were 2.20+0.43 nmol/ml, 547+98 μmol/l, 0.88+0.15 mg/dl and 0.75+0.20 mg/dl respectively in patients. The respective values in controls were 1.86+0.43 nmol/ml, 859+139 μmol/l, 0.94+0.15 mg/dl and 1.10+0.30 mg/dl. Although the OS seems to be raised in patients, is practically insufficient to oxidize biomolecules and induce CVDs. Despite vitamin C and E levels being well within normal limits, the TAA was significantly and considerably lower in patients. This is a highly interesting observation suggesting that dietary antioxidants other than these vitamins were preferentially consumed to control OS because procedure for TAA used in this study practically measures only total dietary antioxidants. Conclusion: OS does not appear to be an etiological factor for the cardiovascular diseases; rather slightly raised OS in patients seems to be a consequence. Further the raised OS was not due to lower nutrient antioxidant (vit. C and vit. E) in the local population studied herein. Key words: Oxidative stress, cardiovascular diseases, vitamin C, vitamin E, total antioxidant activity.Publication Two interesting cases of left atrial myxoma(Kathmandu University, 2010) Alurkar, VM; Neupane, SAbstract Atrial myxoma is the most common type of benign primary heart tumours. Most of these tumours are sporadic in origin occurring at 3rd to 6th decade of life, while hereditary forms do exist. They are mostly found in the left atrium followed by right atrium and ventricles. A left atrial myxoma may present with features of mitral valve obstruction, embolic phenomenon or systemic disease. On diagnosis, prompt surgical removal is necessary which usually gives a complete cure, though recurrences have been reported. We hereby present two cases of solitary left atrial myxoma. Key words: atrial myxoma, echocardiography, tumour impaction