Publication: Study of antifungal activity of amphotericin B-lipid formulations with five lipid carriers
| creativeworkseries.issn | ISSN (Print) : 1993-2979 | ISSN (Online) : 1993-2987 | |
| dc.contributor.author | Adhikari, K | |
| dc.date.accessioned | 2026-04-24T07:19:29Z | |
| dc.date.available | 2026-04-24T07:19:29Z | |
| dc.date.issued | 2016 | |
| dc.description | K Adhikari Department of Pharmacology, People's Dental College and Hospital Pvt. Ltd., affiliated with Tribhuvan University, Naya Bazar, Kathmandu, Nepal | |
| dc.description.abstract | Abstract Introduction:Invasive pulmonary aspergillosis (IPA) is a prime cause of morbidity and mortality in immunocompromised individuals, who is undergoing lung transplantation.Mortality among infected patients is high. Death rate in excess of 90% has been shown in study by Richardson. Most of these invasive mould infections are acquired through the respiratory tract.An increased incidence of invasive fungal infection has created major challenges for medical practitioners. Amphotericin B(AmB) has been the drug of choice for the treatment of many fungal infections, and it is still used as a gold standard therapy against invasive fungal infection and most commonly used to treat life-threatening conditions such as cryptococcosis, histoplasmosis, and IPA.Currently, lipid formulations are accepted as a less toxic alternative to the traditional colloidal dispersion. Drug toxicity, the nephrotoxicity of AmB is the major clinical problem, which could lead to treatment discontinuation. We designed five lipid formulations of AmB, and compared the dose-response effects of all lipid formulations in-vitro against three different fungal strains including Cryptococcus neoformans, Candida albicans and Saccharomyces cerevisiae. Methods: Five lipid formulations amphotericin B were formulated by lyophilization process. The cylinder plate method performs the relative potency by determining the clear zone of AmB-lipid formulation in the inoculum medium as compared with a clear zone of AmB standard. Broth microdilution method can be used to measure the minimum inhibitory concentration (MIC). The MIC value is based upon predetermined end point, which may be interpreted as an absence of visible growth in a broth containing known concentration of AmB.The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) were determined using Cryptococcus neoformans ATCC 90113 NS and Candida albicans ATCC 90028. Results: All fiveformulations showed good responses against C. neoformans and Candida albicans, with the MIC and MFC values in the range between 0.16-0.32 µg/ml. The potency of these formulations was equivalent to pure AmB (100%). Conclusions: The results indicate that the investigateded potassium cholate, potassium deoxycholate, sodium deoxycholate sulfate, sodium cholate and sodium deoxycholate may be used as a promising alternative carrier system for amphotericin B. Keywords: Amphotericin B, Cryptococcus neoformans, Candida albicans, Potency, Lipid drug carriers | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14572/5927 | |
| dc.language.iso | en_US | |
| dc.publisher | Institute of Medicine | |
| dc.subject | Amphotericin B | |
| dc.subject | Cryptococcus neoformans | |
| dc.subject | Candida albicans | |
| dc.subject | Potency | |
| dc.subject | Lipid drug carriers | |
| dc.title | Study of antifungal activity of amphotericin B-lipid formulations with five lipid carriers | |
| dc.type | Article | |
| dspace.entity.type | Publication | |
| local.article.type | Original Article | |
| oaire.citation.endPage | 57 | |
| oaire.citation.startPage | 53 | |
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