Publication: Multi-drug Resistant and Extended Spectrum β-lactamase Producing Salmonella Species Isolated from Fresh Chicken Liver Samples
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Kathmandu University
Abstract
ABSTRACT
Background
Emergence of antibiotic resistance among microbes contaminating the fresh meat
products is a global public health concern as they can be easily transmitted to
humans through their consumption and contact.
Objective
The current study was conducted to determine the distribution of antimicrobial
resistance among Salmonella species isolated from fresh chicken liver samples with
special emphasis on extended spectrum beta-lactamase (ESBL) production.
Method
A total of 200 fresh chicken liver samples were cultivated for the isolation of
Salmonella and further subcultivated to detect extended spectrum beta-lactamase
production among them. Antimicrobial susceptibility testing (AST) was done by disk
diffusion method using a panel of 7 antimicrobials.
Result
Out of 200 samples analyzed, 61 (30.5%) samples harbored Salmonella species out
of which 15 (7.5%) samples showed the presence of Salmonella Typhi. A significant
association was noted in the incidence of Salmonella with various factors pertaining to
the butchers, such as age, sex, literacy rate, practices of washing knives and chopping
board, wearing aprons and gloves and type of water used (p < 0.05). Salmonella
isolates were highly sensitive to amikacin (82.0%) and least sensitive to tetracycline
(3.3%). All the isolates were resistant to colistin. Sixty (98.4%) isolates were identified
as multi-drug resistant (MDR). The total number of extended spectrum beta-
lactamase producers reported among Salmonella isolates was 29 (47.5%).
Conclusion
The results indicate that the fresh chicken liver samples sold in Bharatpur Metropolis
are reservoirs of multi-drug resistant Salmonella, including extended spectrum beta-
lactamase producers, that could potentially be transmitted to the humans by direct
contact or through inadequate cooking.
KEY WORDS
Antimicrobial susceptibility testing, Extended spectrum beta-lactamase, Fresh
chicken liver samples, Multi-drug resistant, Salmonella
Description
Adhikari S,1 Khadka S,1,2 Sapkota S,1,3 Sharma BR,1 Ghimire A,1 Chalise M,1 Gurung D,1 Kunwar S1
1Department of Microbiology,
Birendra Multiple Campus,
Tribhuvan University, Bharatpur, Chitwan, 44200, Nepal.
2Department of Biochemistry and
Molecular Biology,
School of Life Sciences,
Central China Normal University,
Wuhan, Hubei, 430079, P.R. China.
3State Key Laboratory of Respiratory Disease,
Guangzhou Institutes of Biomedicine and Health,
Chinese Academy of Sciences,
Guangzhou 510530, P.R. China
Keywords
Antimicrobial susceptibility testing, Extended spectrum beta-lactamase, Fresh chicken liver samples, Multi-drug resistant, Salmonella