Multi-drug Resistant and Extended Spectrum β-lactamase Producing Salmonella Species Isolated from Fresh Chicken Liver Samples

Adhikari, S; Khadka, S; Sapkota, S; Sharma, BR; Ghimire, A; Chalise, M; Gurung, D; Kunwar, S

Publication:
Multi-drug Resistant and Extended Spectrum β-lactamase Producing Salmonella Species Isolated from Fresh Chicken Liver Samples

creativeworkseries.issn	1812-2027
dc.contributor.author	Adhikari, S
dc.contributor.author	Khadka, S
dc.contributor.author	Sapkota, S
dc.contributor.author	Sharma, BR
dc.contributor.author	Ghimire, A
dc.contributor.author	Chalise, M
dc.contributor.author	Gurung, D
dc.contributor.author	Kunwar, S
dc.date.accessioned	2025-12-05T05:31:31Z
dc.date.available	2025-12-05T05:31:31Z
dc.date.issued	2020
dc.description	Adhikari S,1 Khadka S,1,2 Sapkota S,1,3 Sharma BR,1 Ghimire A,1 Chalise M,1 Gurung D,1 Kunwar S1 1Department of Microbiology, Birendra Multiple Campus, Tribhuvan University, Bharatpur, Chitwan, 44200, Nepal. 2Department of Biochemistry and Molecular Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei, 430079, P.R. China. 3State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, P.R. China
dc.description.abstract	ABSTRACT Background Emergence of antibiotic resistance among microbes contaminating the fresh meat products is a global public health concern as they can be easily transmitted to humans through their consumption and contact. Objective The current study was conducted to determine the distribution of antimicrobial resistance among Salmonella species isolated from fresh chicken liver samples with special emphasis on extended spectrum beta-lactamase (ESBL) production. Method A total of 200 fresh chicken liver samples were cultivated for the isolation of Salmonella and further subcultivated to detect extended spectrum beta-lactamase production among them. Antimicrobial susceptibility testing (AST) was done by disk diffusion method using a panel of 7 antimicrobials. Result Out of 200 samples analyzed, 61 (30.5%) samples harbored Salmonella species out of which 15 (7.5%) samples showed the presence of Salmonella Typhi. A significant association was noted in the incidence of Salmonella with various factors pertaining to the butchers, such as age, sex, literacy rate, practices of washing knives and chopping board, wearing aprons and gloves and type of water used (p < 0.05). Salmonella isolates were highly sensitive to amikacin (82.0%) and least sensitive to tetracycline (3.3%). All the isolates were resistant to colistin. Sixty (98.4%) isolates were identified as multi-drug resistant (MDR). The total number of extended spectrum beta- lactamase producers reported among Salmonella isolates was 29 (47.5%). Conclusion The results indicate that the fresh chicken liver samples sold in Bharatpur Metropolis are reservoirs of multi-drug resistant Salmonella, including extended spectrum beta- lactamase producers, that could potentially be transmitted to the humans by direct contact or through inadequate cooking. KEY WORDS Antimicrobial susceptibility testing, Extended spectrum beta-lactamase, Fresh chicken liver samples, Multi-drug resistant, Salmonella
dc.identifier.uri	https://hdl.handle.net/20.500.14572/3385
dc.language.iso	en_US
dc.publisher	Kathmandu University
dc.subject	Antimicrobial susceptibility testing
dc.subject	Extended spectrum beta-lactamase
dc.subject	Fresh chicken liver samples
dc.subject	Multi-drug resistant
dc.subject	Salmonella
dc.title	Multi-drug Resistant and Extended Spectrum β-lactamase Producing Salmonella Species Isolated from Fresh Chicken Liver Samples
dc.type	Article
dspace.entity.type	Publication
local.article.type	Original Article
oaire.citation.endPage	27
oaire.citation.startPage	23
relation.isJournalIssueOfPublication	6a5c6938-6a89-4fe4-9dfc-d3d149832590
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