Publication: Study of Helicobacter hepaticus in gallbladders with cholelithiasis and its sensitivity pattern
Date
2009
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Kathmandu University
Abstract
Abstract
Background: Cholelithiasis is one of the most common digestive surgical disorders. The natural history of gallstone
development is unknown. Bacteria are found in high concentration in bile and stone. It is diffcult to ascertain whether
bacterial infection of the bile arose before stone formation or vice versa.
Materials & methods: Prospective study was carried out in 30 cholecystectomy specimens of patients with cholelithiasis
collected from 15th January 2005 to 24th July 2005 in Department of Pathology, Kathmandu Medical College Teaching
Hospital. The samples were collected in a sterile vial containing 0.5% saline. A small piece of gallbladder was taken in
Blood agar and Chocolate agar media for micro anaerobic culture at 370 C. The growth of the organism was observed after
48 hours of incubation. Gram stain and various biochemical tests were performed for the confirmation of Helicobacter
hepaticus and its sensitivity pattern was studied. Histologically Helicobacter hepaticus was demonstrated in formalin
fixed tissue sections using Warthin Starry Silver stain.
Results: A total of 76.66% cases showed growth positivity. Nitrofurantoin was found to be the most sensitive drug
(95.7%) for Helicobacter hepaticus followed by Ciprofloxacin (91.3%), Cephalaxin (91.3%), Certriaxone (91.3%),
Ofloxacin (82.6%), Amikacin (65.2%) and Norfloxacin (60.9%). Helicobacter hepaticus was least sensitive to
Amoxycillin (57%).
Conclusion: From this study, it is quite apparent that Helicobacter hepaticus infection is commonly associated (76.66%)
with cholelithiasis. If the patients are treated with the sensitive antibiotics regularly and the infection, that is the number one
cause for the precipitation of bile leading to the formation of gallstones, can be controlled, the incidence of cholelithiasis
can be reduced so that the health care expenditure related to gallbladder disease can be reduced markedly.
Key words: Cholelithiasis Helicobacter hepaticus, WSS stain
Description
SB Pradhan
Lecturer, Department of Pathology, Kathmandu Medical College & Teaching Hospital, Sinamangal, Nepal