Publication: The Significance of ERG and Androgen Receptor Expression in Adenocarcinoma Prostate
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Kathmandu University
Abstract
ABSTRACT
Background
Fusions of transmembrane protease, serine 2 (TMPRSS2) with erythroblast
transformation specific transcription factors have been found in prostate cancer. The
v-etserythroblastosis virus E26 oncogene homologue (ERG) is a proto-oncogene of
the erythroblast transformation specific transcription factor family. TMPRSS2-ERG
fusion is the most common molecular alteration present in about 50% of prostatic
adenocarcinomas. Androgen receptor (AR) plays a key role in prostate development
and is involved in the progression of prostate cancer.
Objective
To evaluate the significance of combined ERG and AR expression in cases of prostatic
adenocarcinoma.
Method
The study was conducted at Dr. Ram Manohar Lohia Institute of Medical Sciences,
Lucknow, Uttar Pradesh, India. Formalin fixed-paraffin embedded archival prostatic
tissue specimens were obtained. A total of 10 cases of prostatic adenocarcinoma
were included in the study. Immunohistochemistry for Androgen receptor was done
by the standard protocol. Multiplex immunohistochemical staining was done for
ERG+CK5 using a primary antibody cocktail of mouse and rabbit antibodies.
Result
Specific AR immunostaining was exclusively nuclear and was present in all 10 cases in
varying intensity. Specific ERG immunostaining was nuclear and was present in seven
cases (70%) and absent in three cases (30%). The three cases that were negative for
ERG had a Gleason score of ≤ 6 and the AR staining was strong and present in about
90% of the cells. Gleason score was directly related to the ERG staining while AR
staining was inversely related to the ERG staining.
Conclusion
The prognostic value of combined ERG and AR over-expression, its associated genes
should be further investigated as potential therapeutic targets in prostate cancer
progression. Preliminary data is being presented. Larger prospective studies with
survival analysis are essential for prognostic significance.
KEY WORDS
Androgen Receptor, Prostate Adenocarcinoma, v-etserythroblastosis virus E26
oncogene homologue
Description
Husain I,1 Sagar P,2 Shukla S,3 Babu S,4 Singhai A,4 Sankhwar SN,4 Husain N3
1Era’s Lucknow Medical College and Hospital,
Lucknow, Uttar Pradesh.
2ONGC Hospital,
Dehradun.
3Dr. Ram Manohar lohia,
Institute of Medical Sciences, Lucknow, Uttar Pradesh.
4King George’s Medical University,
Lucknow
Keywords
Androgen Receptor, Prostate Adenocarcinoma, v-etserythroblastosis virus E26 oncogene homologue